The Actinobacteriophage Database

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Detailed Information for Phage Clayda5
Discovery Information
Isolation Host	Microbacterium foliorum NRRL B-24224
Found By	Ashley Van Egdom
Year Found	2021
Location Found	Hull, IA United States
Finding Institution	Northwestern College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	43.191844 N, 96.138591 W Map
Naming Notes	This phage is a combination of my sisters' names and the 5 members of my family.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 16, 2021
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	601
Genome length (bp)	39894
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	ACTCCCGGCA
GC Content	67.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	EB
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Abigail Albedo Albright AnnaLie Armstrong Arroyo AvGardian Avocadoman BabyDaisy BabyYoda Bachaco BAjuniper BelmontSKP Bernstein Brahms BubbaBear Buldak Burritobowl Cashington Celaena ChiliPepper Clayda5 CliffHanger Coltrane CroZenni DickRichards Didgeridoo DirtyBubble Dismas Doobus Eden Elva Eula Finalfrontier FlameThrower Franklin22 Gack Icarian IndyLu Johnathan Jovita Kate33 Katzastrophic Kenzers Kieran Lahqtemish LimaBean Loviatar Lynlen Nicky22 Olliecat Phisb QMacho Quenya Rollins Rona SanaSana SansAfet SarBear Sharkboy Shayna Skylord Slay Squircle Stoor Stromboli SunnyD Swervy Teagster TukTuk Vitas WalkingDead
Annotating Institution	Northwestern College
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	70
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Clayda5_1 (terminase small subunit) Clayda5_2 (terminase large subunit) Clayda5_3 (portal protein) Clayda5_4 (capsid maturation protease) Clayda5_5 (major capsid protein) Clayda5_6 Clayda5_7 (head-to-tail adaptor) Clayda5_8 (head-to-tail stopper) Clayda5_9 Clayda5_10 (tail terminator) Clayda5_11 (major tail protein) Clayda5_12 (tail assembly chaperone) Clayda5_13 (tail assembly chaperone) Clayda5_14 Clayda5_15 (tape measure protein) Clayda5_16 (minor tail protein) Clayda5_17 (minor tail protein) Clayda5_18 (minor tail protein) Clayda5_19 (minor tail protein) Clayda5_20 (endolysin) Clayda5_21 Clayda5_22 Clayda5_23 Clayda5_24 Clayda5_25 Clayda5_26 (Cas4 family exonuclease) Clayda5_27 Clayda5_28 (HicA-like toxin) Clayda5_29 (deoxyuridine triphosphatase) Clayda5_30 (thymidylate kinase) Clayda5_31 (recombination directionality factor) Clayda5_32 Clayda5_33 Clayda5_34 (NrdH-like glutaredoxin) Clayda5_35 (holliday junction resolvase) Clayda5_36 Clayda5_37 Clayda5_38 Clayda5_39 Clayda5_40 (DNA primase) Clayda5_41 (DNA polymerase I) Clayda5_42 Clayda5_43 Clayda5_44 (DNA binding protein) Clayda5_45 Clayda5_46 (SprT-like protease) Clayda5_47 Clayda5_48 Clayda5_49 Clayda5_50 Clayda5_51 (HNH endonuclease) Clayda5_52 Clayda5_53 Clayda5_54 Clayda5_55 Clayda5_56 Clayda5_57 Clayda5_58 (dihydrofolate reductase) Clayda5_59 Clayda5_60 Clayda5_61 (thymidylate synthase) Clayda5_62 Clayda5_63 Clayda5_64 Clayda5_65 Clayda5_66 Clayda5_67 Clayda5_68 Clayda5_69 Clayda5_70 — tRNA (tRNA-Asn(gtt)) Clayda5_71 — tRNA (tRNA-Gln(ctg)) Clayda5_72 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OM818329
Refseq Number	None yet
Published in Paper	Bastian et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Microbacterium Phages Clayda5 and Gshelby23 and Gordonia Phages Wrigley and Santhid
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	137
Pitt Freezer Box Grid#	A4
Available Files
Plaque Picture	Download
GenBank File for Phamerator	Download

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