| Detailed Information for Phage CliffHanger |
| Discovery Information |
| Isolation Host | Curtobacterium flaccumfaciens NRRL B-729 SEA |
| Found By | Dylan Garagozzo |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44177 N, 79.9502 W Map |
| Discovery Notes | Found in soil near the roots of a plant. |
| Naming Notes | Plant was near a bridge close to Schenley on a mini cliff. Dylan had to walk a little bit down said cliff to get the dirt, hence the name. Dylan found this on a walk with his friend Catherine R. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 7083 |
| Genome length (bp) | 41153 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | AGTCCCGGCT |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are observed to be clear and rounded. Plaques are surprisingly big at around 3 mm. |
| Number of Genes | 75 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ868954 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
220 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| GenBank File for Phamerator | Download |