Curtobacterium phage CliffHanger
CliffHanger Plaque Picture
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Detailed Information for Phage CliffHanger
Discovery Information
Isolation HostCurtobacterium flaccumfaciens NRRL B-729 SEA
Found ByDylan Garagozzo
Year Found2024
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.44177 N, 79.9502 W Map
Discovery NotesFound in soil near the roots of a plant.
Naming NotesPlant was near a bridge close to Schenley on a mini cliff. Dylan had to walk a little bit down said cliff to get the dirt, hence the name. Dylan found this on a walk with his friend Catherine R.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 14, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage7083
Genome length (bp)41153
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceAGTCCCGGCT
GC Content65.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are observed to be clear and rounded. Plaques are surprisingly big at around 3 mm.
Number of Genes75
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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CliffHanger_1 — tRNA
(tRNA-Lys(ctt))

CliffHanger_2 — tRNA
(tRNA-Trp(cca))

CliffHanger_3
(terminase, small subunit)

CliffHanger_4
(terminase, large subunit)

CliffHanger_5
(portal protein)

CliffHanger_6
(capsid maturation protease)

CliffHanger_7
(major capsid protein)

CliffHanger_8

CliffHanger_9
(head-to-tail adaptor)

CliffHanger_10
(head-to-tail stopper)

CliffHanger_11

CliffHanger_12
(tail terminator)

CliffHanger_13
(major tail protein)

CliffHanger_14
(tail assembly chaperone)

CliffHanger_15
(tail assembly chaperone)

CliffHanger_16
(tape measure protein)

CliffHanger_17
(minor tail protein)

CliffHanger_18
(minor tail protein)

CliffHanger_19
(minor tail protein)

CliffHanger_20
(minor tail protein)

CliffHanger_21

CliffHanger_22

CliffHanger_23

CliffHanger_24
(endolysin)

CliffHanger_25

CliffHanger_26

CliffHanger_27

CliffHanger_28

CliffHanger_29

CliffHanger_30
(Cas4 family exonuclease)

CliffHanger_31

CliffHanger_32

CliffHanger_33

CliffHanger_34
(MazG-like nucleotide pyrophosphohydrolase)

CliffHanger_35
(thymidylate kinase)

CliffHanger_36
(recombination directionality factor)

CliffHanger_37
(NrdH-like glutaredoxin)

CliffHanger_38
(Holliday junction resolvase)

CliffHanger_39

CliffHanger_40

CliffHanger_41

CliffHanger_42

CliffHanger_43
(DNA primase/helicase)

CliffHanger_44
(DNA polymerase I)

CliffHanger_45

CliffHanger_46

CliffHanger_47
(DNA binding protein)

CliffHanger_48

CliffHanger_49

CliffHanger_50

CliffHanger_51
(HNH endonuclease)

CliffHanger_52

CliffHanger_53

CliffHanger_54

CliffHanger_55

CliffHanger_56

CliffHanger_57

CliffHanger_58

CliffHanger_59

CliffHanger_60

CliffHanger_61

CliffHanger_62

CliffHanger_63

CliffHanger_64

CliffHanger_65

CliffHanger_66

CliffHanger_67

CliffHanger_68

CliffHanger_69
(thymidylate synthase)

CliffHanger_70

CliffHanger_71

CliffHanger_72

CliffHanger_73

CliffHanger_74

CliffHanger_75

CliffHanger_76

CliffHanger_77 — tRNA
(tRNA-Asn(gtt))

CliffHanger_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ868954
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
GenBank File for PhameratorDownload