| Detailed Information for Phage CroZenni |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Elle Zenni |
| Year Found | 2019 |
| Location Found | Cleves, Ohio, OH United States of America |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.156389 N, 84.726944 W Map |
| Discovery Notes | Found on the side of the road in dirt at a construction site in a neighborhood. The soil was dry, sandy, and in the sunlight. The temperature at the time of sample collection was about 75°F. |
| Naming Notes | A combination of my name and my partner's name, Luke Croner. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 162 |
| Genome length (bp) | 41471 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | appears to be partially lysogenic due to the halos surrounding the plaques |
| Other Cluster Members |
|
| Annotating Institution | Miami University |
| Annotation Status | In GenBank |
| Plaque Notes | medium sized, circular plaques with well defined, clear centers and halos surrounding them |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712721 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
107 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |