| Detailed Information for Phage Didgeridoo |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gabrielle Gentile |
| Year Found | 2016 |
| Location Found | Wheeling , WV USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.047953 N, 80.66808 W Map |
| Discovery Notes | This sample was taken from a puddle behind a dugout at the baseball fields. The soil was firm and brown in color with an orangish tint. The weather was cloudy. Sample was collected on 5.4 at 5:45 PM. |
| Naming Notes | Named after the Australian musical instrument.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 1, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3125 |
| Genome length (bp) | 42655 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGACA |
| End Determination Notes | Build up of reads on opposite strands separated by 10 bp. |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Didgeridoo forms large plaques that are clear and approximately 3mm in size. |
| Number of Genes | 74 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH045566 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
E6 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |