Microbacterium phage Didgeridoo
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Detailed Information for Phage Didgeridoo
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByGabrielle Gentile
Year Found2016
Location FoundWheeling , WV USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.047953 N, 80.66808 W Map
Discovery NotesThis sample was taken from a puddle behind a dugout at the baseball fields. The soil was firm and brown in color with an orangish tint. The weather was cloudy. Sample was collected on 5.4 at 5:45 PM.
Naming NotesNamed after the Australian musical instrument.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 1, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3125
Genome length (bp)42655
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceACTCCCGACA
End Determination NotesBuild up of reads on opposite strands separated by 10 bp.
GC Content66.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesDidgeridoo forms large plaques that are clear and approximately 3mm in size.
Number of Genes74
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Didgeridoo_1

Didgeridoo_2

Didgeridoo_3
(terminase small subunit)

Didgeridoo_4
(terminase)

Didgeridoo_5
(portal protein)

Didgeridoo_6
(capsid maturation protease)

Didgeridoo_7
(major capsid protein)

Didgeridoo_8

Didgeridoo_9
(head-to-tail adaptor)

Didgeridoo_10
(head-to-tail stopper)

Didgeridoo_11

Didgeridoo_12
(tail terminator)

Didgeridoo_13

Didgeridoo_14
(tail assembly chaperone)

Didgeridoo_15
(tail assembly chaperone)

Didgeridoo_77

Didgeridoo_17
(tape measure protein)

Didgeridoo_18
(minor tail protein)

Didgeridoo_19
(minor tail protein)

Didgeridoo_20

Didgeridoo_21

Didgeridoo_22

Didgeridoo_23

Didgeridoo_24

Didgeridoo_25

Didgeridoo_26

Didgeridoo_28

Didgeridoo_29

Didgeridoo_30
(Cas4 family exonuclease)

Didgeridoo_31
(HNH endonuclease)

Didgeridoo_32

Didgeridoo_33
(deoxyuridine triphosphatase)

Didgeridoo_34
(thymidylate kinase)

Didgeridoo_35
(recombination directionality factor)

Didgeridoo_36

Didgeridoo_37

Didgeridoo_38
(NrdH-like glutaredoxin)

Didgeridoo_39
(holliday junction resolvase)

Didgeridoo_40

Didgeridoo_41

Didgeridoo_42

Didgeridoo_43

Didgeridoo_44
(DNA primase/helicase)

Didgeridoo_45
(DNA polymerase I)

Didgeridoo_46

Didgeridoo_47

Didgeridoo_48
(DNA binding protein)

Didgeridoo_49

Didgeridoo_50

Didgeridoo_51

Didgeridoo_52
(ParB-like nuclease domain protein)

Didgeridoo_53

Didgeridoo_54

Didgeridoo_55
(HNH endonuclease)

Didgeridoo_56

Didgeridoo_57

Didgeridoo_58

Didgeridoo_59

Didgeridoo_60

Didgeridoo_61
(dihydrofolate reductase)

Didgeridoo_62

Didgeridoo_63

Didgeridoo_64
(thymidylate synthase)

Didgeridoo_65

Didgeridoo_66

Didgeridoo_67

Didgeridoo_68

Didgeridoo_69

Didgeridoo_70

Didgeridoo_71

Didgeridoo_72

Didgeridoo_73

Didgeridoo_74 — tRNA
(tRNA-Gln(ctg))

Didgeridoo_75
(HNH endonuclease)

Didgeridoo_76

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH045566
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 5
Pitt Freezer Box Grid# E6
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload