| Detailed Information for Phage DirtyBubble |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Eddie Muschio and Andrew Moonan |
| Year Found | 2018 |
| Location Found | Walden, NY USA |
| Finding Institution | Mount Saint Mary College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.571695 N, 74.204024 W Map |
| Discovery Notes | Found it in the backyard of my house |
| Naming Notes | Name came from an episode of Spongebob, since the plates had the same color and smell of the Dirty Bubble |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1312 |
| Genome length (bp) | 41692 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Mount Saint Mary College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are very small when comparing to others in the class. They are also very irregular in shape, and not a nice defined circle. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN062714 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.29x10^11 |
| Pitt Freezer Box# |
80 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |