| Detailed Information for Phage Doobus |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | David Ennest |
| Year Found | 2018 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.851966 N, 92.232589 W Map |
| Discovery Notes | Found on 9-19-2018.
The soil was collected next to the Kinnickinnic river near the bridge on the trail that connects the falcon trail to the rest of campus.
Weather conditions when collected were clear with low humidity, and the temperature was 72°F.
The soil looked loose, dark, and very moist. It was collected at a depth of 25mm
Coordinates of collection site are : 44.851966 N, 92.232589 W |
| Naming Notes | The name is a conjunction of the words darn and goobus. This is because the phage is as weak as a darn goobus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 18, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 41 |
| Genome length (bp) | 41195 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Queens University of Charlotte |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are minuscule in size and have very slight turbid halos.
Approximate size is that of a written period.
Uniform in size and shape |
| Number of Genes | 68 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ820083 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
87 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
