| Detailed Information for Phage Eden |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kat Hebert, Dakota Archambault, and John Massaad |
| Year Found | 2017 |
| Location Found | Orono, ME USA |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.897522 N, 68.667564 W Map |
| Discovery Notes | The soil was dark in color and moist when collected. |
| Naming Notes | We found it in a garden on the University of Maine campus- the garden of Eden pun was too much to pass up! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 7, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 7600 |
| Genome length (bp) | 40833 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGGCA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | Both turbid and clear plaques occur on a lawn of M. foliorum. The clear plaques measure 2 mm in diameter and the turbid plaques measure 1 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH509447 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
73 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
