| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jaemie Bennett |
| Year Found | 2017 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.33417 N, 76.62361 W Map |
| Discovery Notes | About five feet away and up a slop from Story Run, a rocky, polluted creek. Soil collected from in between roots, near the base of a tree about a foot in diameter. Soil was relatively moist with a few small rocks. It found at 84 F and it was 2:55 pm ET |
| Naming Notes | My phage had difficult and unique morphologies, so a used a (rude) Chinese word, "e jie." I then tacked on my sister's Chinese name because she wanted to, which is "mei xi." So it was "e jie mei xi," which I abbreviated to "EJMX," which became the name "Ejimix." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 920 |
| Genome length (bp) | 111924 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Johns Hopkins University |
| Annotation Status | In GenBank |
| Plaque Notes | My phage actually had two morphologies, but I ran extensive generations of dilutions to prove it was one phage. There was small form of the plaque, which were about a millimeter in diameter. The large form was about 4 mm in diameter. Generally, the small plaques resulted from a high-concentrated plate, and the large plaques from low concentrations, but on low concentrations there were generally small plaques as well. When the plaques were large enough to tell, there was a cloudy ring around a clear inner circle. |
| Morphotype | Siphoviridae |
| Number of Genes | 234 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH697582 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
70 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
