| Detailed Information for Phage Eula |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Travis Cook |
| Year Found | 2021 |
| Location Found | Statesville, NC United States |
| Finding Institution | Mitchell Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.799806 N, 80.890146 W Map |
| Discovery Notes | Collected from the soil within a chicken coop; sample collected from a depth of 3 centimeters; the temperature outside was 88 degrees Fahrenheit; the moisture content of the soil was moist |
| Naming Notes | The name was derived from the name of the grandmother of one of the Biology professors |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 993 |
| Genome length (bp) | 41379 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Mitchell Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques varied in size with a majority of the plaques appearing fairly large; the plaque numbers ranged from 17 to 200+ depending on the petri dish |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON724018 |
| Refseq Number | None yet |
| Published in Paper | Cook et al 2022, Microbiology Resource Announcements: Complete Genome Sequence of Bacteriophage Eula, Isolated on Microbacterium foliorum |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
132 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
