| Detailed Information for Phage Fregley |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alexis Thomas |
| Year Found | 2022 |
| Location Found | Amherst, MA United States |
| Finding Institution | University of Massachusetts Amherst |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.388053 N, 72.527593 W Map |
| Discovery Notes | Near Herter Annex dirt patch. Not near any trees but bushes were present |
| Naming Notes | Diary of a Wimpy Kid |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 31, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1692 |
| Genome length (bp) | 62291 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 203 bp |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are small, circular (round edges), and partially clear/ translucent and cloudy throughout the plaque. Most of the plaques were 1-2 mm but we chose plaques that were about 2-3mm in diameter
About 15 plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 106 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553896 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
158 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
