| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gabrielle Stack |
| Year Found | 2023 |
| Location Found | Willington, CT United States |
| Finding Institution | University of Connecticut |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.83765 N, 72.262379 W Map |
| Discovery Notes | Found by dumpster near apartment complex - compact soil that is consistently dry and in the shade |
| Naming Notes | The name of this plaque originally came from a combination of my first and last name, however, the morphology of this plaque has an irregular edge, similar to a paint splatter, or goop, something that would make you exclaim GACK! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1387 |
| Genome length (bp) | 40547 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGGCA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | We conducted a lysogeny test and it was found that this phage is lysogenic |
| Other Cluster Members |
|
| Annotating Institution | University of Connecticut |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology is different at different dilutions, with larger cloudy clearings appearing around the irregular center clearings at higher dilutions. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978884 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
183 |
| Pitt Freezer Box Grid# |
A6 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
