Microbacterium phage Icarian
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Detailed Information for Phage Icarian
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByNatalie Vergara
Year Found2023
Location FoundPort saint Lucie, FL United States
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates27.221389 N, 80.3875 W Map
Discovery NotesFound under my grandmother's papaya tree on a humid summer afternoon, 2 inches under the base of the tree. The soil was dry and almost sand like.
Naming NotesIn reference to the Greek myth of Icarus who flew too close to the sun.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 27, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage902
Genome length (bp)42320
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionIndian River State College
Annotation StatusIn GenBank
Plaque NotesThe plaques were small and circular in shape.
MorphotypeSiphoviridae
Number of Genes69
Number of tRNAs4
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Icarian_1 — tRNA
(tRNA-Tyr(gta))

Icarian_2

Icarian_3
(terminase small subunit)

Icarian_4
(terminase large subunit)

Icarian_5
(portal protein)

Icarian_6
(capsid maturation protease)

Icarian_7
(major capsid protein)

Icarian_8

Icarian_9
(head-to-tail adaptor)

Icarian_10
(head-to-tail stopper)

Icarian_11

Icarian_12
(tail terminator)

Icarian_13
(major tail protein)

Icarian_14
(tail assembly chaperone)

Icarian_15
(tail assembly chaperone)

Icarian_16

Icarian_17
(tape measure protein)

Icarian_18
(minor tail protein)

Icarian_19
(minor tail protein)

Icarian_20
(minor tail protein)

Icarian_21
(minor tail protein)

Icarian_22
(endolysin)

Icarian_23

Icarian_24

Icarian_25

Icarian_26

Icarian_27

Icarian_28

Icarian_29

Icarian_30
(Cas4 exonuclease)

Icarian_31

Icarian_32

Icarian_33

Icarian_34
(HicA-like toxin in toxin/antitoxin system)

Icarian_35
(dUTPase)

Icarian_36
(thymidylate kinase)

Icarian_37
(recombination directionality factor)

Icarian_38

Icarian_39
(NrdH-like glutaredoxin)

Icarian_40
(Holliday junction resolvase)

Icarian_41

Icarian_42

Icarian_43

Icarian_44
(DNA primase/helicase)

Icarian_45
(DNA polymerase I)

Icarian_46

Icarian_47

Icarian_48
(endonuclease VII)

Icarian_49
(DNA binding protein)

Icarian_50

Icarian_51

Icarian_52
(ParB-like nuclease domain)

Icarian_53

Icarian_54

Icarian_55
(HNH endonuclease)

Icarian_56

Icarian_57

Icarian_58

Icarian_59

Icarian_60
(dihydrofolate reductase)

Icarian_61
(thymidylate synthase)

Icarian_62

Icarian_63

Icarian_64

Icarian_65

Icarian_66

Icarian_67

Icarian_68

Icarian_69 — tRNA
(tRNA-Asn(gtt))

Icarian_70 — tRNA
(tRNA-Gln(ctg))

Icarian_71 — tRNA
(tRNA-Lys(ctt))

Icarian_72
(HNH endonuclease)

Icarian_73

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978870
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 169
Pitt Freezer Box Grid# D4
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload