| Detailed Information for Phage Johnathan |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Mackenzie Horutz |
| Year Found | 2021 |
| Location Found | WI Kenosha |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 24°C |
| GPS Coordinates | 42.6201 N, 87.8218 W Map |
| Discovery Notes | The soil sample was originally found by Logan Seery, and I adopted a phage from one of his initial plates because my sample did not yield any results. I was partially assisted by Jeanelle Young for the lab procedures. |
| Naming Notes | In all honesty, I did not know what to name my phage. I decided to go with something random, and picked what Paul Rudd said the name of Thor's hammer in an interview once. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 697 |
| Genome length (bp) | 40971 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Carthage College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were of a consistent small size and were rounded in shape. |
| Number of Genes | 68 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP405128 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| GenBank File for Phamerator | Download |