| Detailed Information for Phage KillerQueen |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Charlotte DeLong |
| Year Found | 2024 |
| Location Found | Oxford, OH United States |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.506568 N, 84.727054 W Map |
| Discovery Notes | This sample was found next to a pond. It was collected around 9 a.m., and the temperature was around 50 degrees Fahrenheit. The weather was dry, cool, and partly cloudy. The dirt this phage was isolated from was full of leaves and twigs, and was dry. |
| Naming Notes | This phage is named after both the song "Killer Queen" by the band Queen, and the stand Killer Queen from JoJo's Bizarre Adventure. The stand Killer Queen utilizes bombs to eliminate its opponents, similar to how phages lyse or "blow up" their virus opponents. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 23, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 308 |
| Genome length (bp) | 62907 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 203 bp |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Miami University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques for this phage are very clear and numerous. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915811 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.8*10^9 |
| Pitt Freezer Box# |
198 |
| Pitt Freezer Box Grid# |
B10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
