The Actinobacteriophage Database

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Detailed Information for Phage Klein
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Veronica Smith
Year Found	2011
Location Found	Radnor, PA USA
Finding Institution	Cabrini University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	40.055400 N, 75.374100 W Map
Discovery Notes	found 1 inch deep in damp soil
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 17, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1897
Genome length (bp)	112329
Character of genome ends	3' Sticky Overhang
Overhang Length	4 bases
Overhang Sequence	ATCC
GC Content	60.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	J
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Ariel Bagrid BAKA Beem Bobby Bombitas Constella Courthouse Dallas DmpstrDiver Dove Duke13 Ejimix EricMillard Gonephishing Halley Hannaconda HokkenD Hughesyang JuicyJay Kalah2 KashFlow Klein LittleE Lucky2013 MiaZeal Minerva NihilNomen Odette Omega Optimus Phoebus Porcelain Pound Redno2 Schatzie Squint Superphikiman Thibault ThreeRngTarjay Wanda Yeet Zelink
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	0.3cm plaques grew after 48 hours at 37°C
Morphotype	Siphoviridae
Number of Genes	248
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Klein_1 (HNH endonuclease) Klein_2 (terminase) Klein_3 Klein_4 Klein_5 (HNH endonuclease) Klein_6 Klein_7 Klein_8 Klein_9 Klein_10 (terminase) Klein_11 Klein_12 (portal protein) Klein_13 (capsid maturation protease) Klein_14 (major capsid protein) Klein_15 (glycosyltransferase) Klein_16 (serine/threonine kinase) Klein_17 (methyltransferase) Klein_18 (glycosyltransferase) Klein_19 (glycosyltransferase) Klein_20 (glycosyltransferase) Klein_21 Klein_22 (glycosyltransferase) Klein_23 Klein_24 Klein_25 (head-to-tail adaptor) Klein_26 (head-to-tail stopper) Klein_27 Klein_28 (tail terminator) Klein_29 Klein_30 Klein_31 (major tail protein) Klein_32 (tail assembly chaperone) Klein_33 (tail assembly chaperone) Klein_34 (tape measure protein) Klein_35 Klein_36 (minor tail protein) Klein_37 (minor tail protein) Klein_38 (minor tail protein) Klein_39 (minor tail protein) Klein_40 (minor tail protein) Klein_41 Klein_42 Klein_43 Klein_44 Klein_45 Klein_46 Klein_47 (lysin A) Klein_48 Klein_49 (lysin B) Klein_50 (holin) Klein_51 Klein_52 (thioredoxin) Klein_53 Klein_54 Klein_55 Klein_56 Klein_57 Klein_58 Klein_59 (Lsr2-like DNA bridging protein) Klein_60 Klein_61 Klein_62 Klein_63 Klein_64 Klein_65 Klein_66 Klein_67 Klein_68 Klein_69 Klein_70 Klein_71 Klein_72 Klein_73 Klein_74 Klein_75 Klein_76 Klein_77 Klein_78 Klein_79 Klein_80 (excise) Klein_81 (tyrosine integrase) Klein_82 Klein_83 Klein_84 Klein_85 Klein_86 Klein_87 Klein_88 Klein_89 Klein_90 Klein_91 Klein_92 Klein_93 Klein_94 Klein_95 Klein_96 Klein_97 (helix-turn-helix DNA binding protein) Klein_98 Klein_99 Klein_100 Klein_101 (esterase) Klein_102 Klein_103 Klein_104 Klein_105 Klein_106 Klein_107 Klein_108 Klein_109 Klein_110 Klein_111 Klein_112 Klein_113 Klein_114 Klein_115 (RecB-like exonuclease) Klein_116 Klein_117 Klein_118 (DNA methyltransferase) Klein_119 (HNH endonuclease) Klein_120 Klein_121 (tRNA nucleotidyltransferase) Klein_122 Klein_123 Klein_124 (polynucleotide kinase) Klein_125 Klein_126 Klein_127 (endonuclease VII) Klein_128 (WhiB family transcription factor) Klein_129 Klein_130 Klein_131 Klein_132 Klein_133 Klein_134 (DNA primase/polymerase) Klein_135 Klein_136 Klein_137 Klein_138 Klein_139 Klein_140 Klein_141 Klein_142 (helix-turn-helix DNA binding protein) Klein_143 Klein_144 Klein_145 Klein_146 (helix-turn-helix DNA binding protein) Klein_147 (HNH endonuclease) Klein_148 (methyltransferase) Klein_149 Klein_150 Klein_151 Klein_152 (HNH endonuclease) Klein_153 (methyltransferase) Klein_154 Klein_155 Klein_156 Klein_157 (ClpP-like protease) Klein_158 (DnaQ-like DNA polymerase III subunit) Klein_159 Klein_160 Klein_161 Klein_162 Klein_163 Klein_164 Klein_165 Klein_166 Klein_167 (PAPS reductase-like protein) Klein_168 (nucleotide pyrophosphohydrolase) Klein_169 Klein_170 Klein_171 Klein_172 Klein_173 Klein_174 Klein_175 Klein_176 Klein_177 Klein_178 Klein_179 (metallophosphoesterase) Klein_180 Klein_181 Klein_182 Klein_183 Klein_184 Klein_185 Klein_186 Klein_187 Klein_188 — tRNA (tRNA-Gly(tcc)) Klein_189 Klein_190 Klein_191 (histidine triad nucleotide binding protein) Klein_192 Klein_193 Klein_194 Klein_195 Klein_196 Klein_197 Klein_198 Klein_199 Klein_200 Klein_201 Klein_202 Klein_203 (FtsK-like DNA translocase) Klein_204 Klein_205 Klein_206 Klein_207 Klein_208 (Ku-like dsDNA break-binding protein) Klein_209 Klein_210 Klein_211 Klein_212 Klein_213 Klein_214 Klein_215 Klein_216 Klein_217 Klein_218 Klein_219 Klein_220 Klein_221 Klein_222 Klein_223 Klein_224 Klein_225 Klein_226 Klein_227 Klein_228 Klein_229 Klein_230 Klein_231 Klein_232 Klein_233 Klein_234 Klein_235 Klein_236 Klein_237 Klein_238 Klein_239 Klein_240 Klein_241 Klein_242 Klein_243 Klein_244 Klein_245 Klein_246 Klein_247 Klein_248 Klein_249
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919512
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2011CABRkleinVS
SEA Lysate Titer	2.07e9
Pitt Freezer Box#	22
Pitt Freezer Box Grid#	68
Available Files
Restriction Digest Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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