| Detailed Information for Phage LuckyBarnes |
| Discovery Information |
| Isolation Host | Brevibacterium iodinum ATCC 15728 |
| Found By | Savannah Underwood |
| Year Found | 2016 |
| Location Found | D'Iberville, MS USA |
| Finding Institution | University of Southern Mississippi |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.439762 N, 88.91651 W Map |
| Discovery Notes | Soil sample was taken from a shaded area with dark, soft soil that had received heavy rainfall in the last week. There was little vegetation in the area. |
| Naming Notes | I was going to name him BuckyBarnes after my recently deceased goldfish, but the name was ccopyrighted so I had to improvise. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 7, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3954 |
| Genome length (bp) | 50774 |
| Character of genome ends | Unknown |
| GC Content | 61.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are about 1 mm in diameter, very clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF668275 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.1x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Nov 21, 2016 |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
