| Detailed Information for Phage Marleymoo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Grace Sebestyen |
| Year Found | 2024 |
| Location Found | harrisonburg, VA united states |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.43439 N, 78.86047 W Map |
| Discovery Notes | It was a hot sunny day outside and I found the phage in a plant box outside of the bio science building at JMU that was watered that morning. |
| Naming Notes | I named my phage after my dog Marley Moo |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1231 |
| Genome length (bp) | 108358 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | James Madison University |
| Annotation Status | Being Annotated (Expected completion by 12/31/2025) |
| Plaque Notes | My plaques are fairly small, especially in my flooded plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 219 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |