| Detailed Information for Phage RubyRalph |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Found By | Lexia Sakowski and Sena Sperloen |
| Year Found | 2018 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.852 N, 92.6187 W Map |
| Discovery Notes | Soil sample from Sena Sperloen
Soil was collected on 9/9/2018 at 7:37pm. It was collected on a trail by the river/bridge by central building. The soil was loose, slightly moist, but mostly dry. It was dugged up 3.5 inches and 65 degrees F. It was cloudy and cool with lots of vegetation around. |
| Naming Notes | It is named after my dog, Ruby. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 19, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 144 |
| Genome length (bp) | 61880 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 209 bp |
| GC Content | 69.0% |
| Sequencing Notes | This phage was sequenced as part of a DOGEMS sample, then the sequence was matched to the lysate by PCR. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | The size of the phage is extremely tiny. They were clear with no halo. It was uniformed in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN908694 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
88 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
