Microbacterium phage SanaSana
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Detailed Information for Phage SanaSana
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByJoseph Sabbagh
Year Found2022
Location FoundPort Saint Lucie, FL United States
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates27.270556 N, 80.346389 W Map
Discovery NotesSample was found in a compost bin. The bin was comprised of paper, cardboard, fruits, vegetable, egg shells, and coffee grounds. The sample was both dense and had trace amounts of liquid. The bin faces the sun during during about half of the day.
Naming NotesThe name was chosen from an old rhyme my mother used to say when I got hurt as a child. Make it both nostalgic as well as a reminder that with time things heal.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage208
Genome length (bp)42006
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content69.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionIndian River State College
Annotation StatusIn GenBank
Plaque NotesThe plaques were of varying size, mostly being comprised of medium to larger plaques.
Number of Genes68
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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SanaSana_1 — tRNA
(tRNA-Tyr(gta))

SanaSana_2

SanaSana_3
(terminase small subunit)

SanaSana_4
(terminase large subunit)

SanaSana_5
(portal protein)

SanaSana_6
(capsid maturation protease)

SanaSana_7
(major capsid protein)

SanaSana_8

SanaSana_9
(head-to-tail adaptor)

SanaSana_10
(head-to-tail stopper)

SanaSana_11

SanaSana_12
(tail terminator)

SanaSana_13
(major tail protein)

SanaSana_14
(tail assembly chaperone)

SanaSana_15
(tail assembly chaperone)

SanaSana_16

SanaSana_17
(tape measure protein)

SanaSana_18
(minor tail protein)

SanaSana_19
(minor tail protein)

SanaSana_20
(minor tail protein)

SanaSana_21
(minor tail protein)

SanaSana_22
(endolysin)

SanaSana_23

SanaSana_24

SanaSana_25

SanaSana_26

SanaSana_27

SanaSana_28

SanaSana_29

SanaSana_30
(Cas4 exonuclease)

SanaSana_31

SanaSana_32

SanaSana_33

SanaSana_34
(HicA-like toxin)

SanaSana_35
(deoxyuridine triphosphatase)

SanaSana_36
(thymidylate kinase)

SanaSana_37
(recombination directionality factor)

SanaSana_38

SanaSana_39
(NrdH-like glutaredoxin)

SanaSana_40
(Holliday junction resolvase)

SanaSana_41

SanaSana_42

SanaSana_43

SanaSana_44
(DNA primase/helicase)

SanaSana_45
(DNA polymerase I)

SanaSana_46

SanaSana_47

SanaSana_48
(DNA binding protein)

SanaSana_49

SanaSana_50

SanaSana_51
(ParB-like nuclease domain protein)

SanaSana_52

SanaSana_53

SanaSana_54
(HNH endonuclease)

SanaSana_55

SanaSana_56

SanaSana_57

SanaSana_58

SanaSana_59
(dihydrofolate reductase)

SanaSana_60
(thymidylate synthase)

SanaSana_61

SanaSana_62

SanaSana_63

SanaSana_64

SanaSana_65

SanaSana_66

SanaSana_67

SanaSana_68 — tRNA
(tRNA-Asn(gtt))

SanaSana_69 — tRNA
(tRNA-Gln(ctg))

SanaSana_70
(HNH endonuclease)

SanaSana_71

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP059646
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 169
Pitt Freezer Box Grid# C12
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload