| Detailed Information for Phage SansAfet |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Leign-DeVito Johnson |
| Year Found | 2018 |
| Location Found | Gloucester County, VA USA |
| Finding Institution | Virginia State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.408818 N, 76.581163 W Map |
| Discovery Notes | SansAfet was discovered in the middle of a walkway of my classmate's vegetable garden. It had also rained two days before this phage sample was collected. It was also adopted during the semester. SansAfet creates medium-sized, non-turbid plaques. |
| Naming Notes | SansAfet got half its name from the brother of my classmate who went on an adventure with her to discover it. The name SansAfet has a Latin prefix and a German suffix. 'Sans' meaning 'without' and 'Afet' meaning 'disaster' due to the ease it allowed me when conducting protocols. SansAfet appears in small and medium morphologies with broad halos surrounding it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 1, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 18 |
| Genome length (bp) | 41531 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Virginia State University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN329675 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
79 |
| Pitt Freezer Box Grid# |
F5 |
| Available Files |
| GenBank File for Phamerator | Download |