Microbacterium phage Skylord
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Detailed Information for Phage Skylord
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found BySkylar Wilkerson
Year Found2020
Location FoundRancho Cucamonga, CA United States
Finding InstitutionLa Sierra University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates34.15 N, 117.52 W Map
Discovery Notesthe isolation sample was taken from my front yard on January 8th at about 1:45 pm. I dug about an inch deep and the soil was dark and damp, there were organic materials such as twigs and rock included in the sample.
Naming Noteswhen I was younger my little cousins and i had nicknames that only we would call each other. The one cousin I am closest with gave me the nickname, Skylord, and he still calls me this to this day
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1720
Genome length (bp)39843
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceACTCCCGGCA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionLa Sierra University
Annotation StatusIn GenBank
Plaque Notessmall, clear, lytic plaques
MorphotypeSiphoviridae
Number of Genes68
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Skylord_1
(terminase small subunit)

Skylord_2
(terminase large subunit)

Skylord_3
(portal protein)

Skylord_4
(capsid maturation protease)

Skylord_5
(major capsid protein)

Skylord_6

Skylord_7
(head-to-tail adaptor)

Skylord_8
(head-to-tail stopper)

Skylord_9

Skylord_10
(tail terminator)

Skylord_11
(major tail protein)

Skylord_12
(tail assembly chaperone)

Skylord_13
(tail assembly chaperone)

Skylord_14

Skylord_15
(tape measure protein)

Skylord_16
(minor tail protein)

Skylord_17
(minor tail protein)

Skylord_18
(minor tail protein)

Skylord_19
(minor tail protein)

Skylord_20
(endolysin)

Skylord_21

Skylord_22

Skylord_23

Skylord_24

Skylord_25
(Cas4 family exonuclease)

Skylord_26

Skylord_27
(HicA-like toxin)

Skylord_28
(deoxyuridine triphosphatase)

Skylord_29
(thymidylate kinase)

Skylord_30

Skylord_31

Skylord_32

Skylord_33
(NrdH-like glutaredoxin)

Skylord_34
(holliday junction resolvase)

Skylord_35

Skylord_36

Skylord_37

Skylord_38

Skylord_39
(DNA primase/helicase)

Skylord_40
(DNA polymerase I)

Skylord_41

Skylord_42

Skylord_43
(DNA binding protein)

Skylord_44

Skylord_45
(SprT-like protease)

Skylord_46

Skylord_47

Skylord_48

Skylord_49
(HNH endonuclease)

Skylord_50

Skylord_51

Skylord_52

Skylord_53

Skylord_54

Skylord_55

Skylord_56
(dihydrofolate reductase)

Skylord_57

Skylord_58

Skylord_59
(thymidylate synthase)

Skylord_60

Skylord_61

Skylord_62

Skylord_63

Skylord_64

Skylord_65

Skylord_66

Skylord_67

Skylord_68 — tRNA
(tRNA-Asn(gtt))

Skylord_69 — tRNA
(tRNA-Gln(ctg))

Skylord_70
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOK999981
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 120
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload