| Detailed Information for Phage Skylord |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Skylar Wilkerson |
| Year Found | 2020 |
| Location Found | Rancho Cucamonga, CA United States |
| Finding Institution | La Sierra University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 34.15 N, 117.52 W Map |
| Discovery Notes | the isolation sample was taken from my front yard on January 8th at about 1:45 pm. I dug about an inch deep and the soil was dark and damp, there were organic materials such as twigs and rock included in the sample. |
| Naming Notes | when I was younger my little cousins and i had nicknames that only we would call each other. The one cousin I am closest with gave me the nickname, Skylord, and he still calls me this to this day |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1720 |
| Genome length (bp) | 39843 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGGCA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | La Sierra University |
| Annotation Status | In GenBank |
| Plaque Notes | small, clear, lytic plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK999981 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
120 |
| Pitt Freezer Box Grid# |
B12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
