Microbacterium phage ValentiniPuff
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| Detailed Information for Phage ValentiniPuff | |
| Discovery Information | |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Found By | Kylie Myers |
| Year Found | 2017 |
| Location Found | Fairchild, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.637839 N, 91.000214 W Map |
| Discovery Notes | found in grassy area where cows eat soil condition: little moist, clumpy, and dark |
| Naming Notes | Named after my favorite saltwater fish a cute Valentini puffer fish |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 8, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 898 |
| Genome length (bp) | 62517 |
| Character of genome ends | Circularly Permuted |
| End Determination Notes | sequence was cut at the first place upstream of the terminase |
| GC Content | 67.4% |
| Sequencing Notes | The assembly contains some mixed reads that align to the phage sequence and some other sequence (possible host DNA). The assembly also contains some contigs that blast to Microbacterium plasmid. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | size and shape: uniform description: turbid with halo fading |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825712 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 70 |
| Pitt Freezer Box Grid# | A1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
