| Detailed Information for Phage Wanda |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | James Bowen |
| Year Found | 2011 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.604722 N, 75.375277 W Map |
| Discovery Notes | Soil was from a large sink hole opened up from Hurricane Irene. Depth about 2 feet. Soil was neither very moist nor very dry. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 24, 2012 |
| Sequencing Facility | David H. Murdock Research Institute |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 252 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 109960 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | "Bulls Eye". Very small (1-2 mm) after 24 hours. After 48 hours plaques usually develop Halo-effect with turbid-edges. After 48 hours of incubation plaques are between 4-5 mm. If plaques are very crowded then bulls-eyes seem to develop at 48 hours too. |
| Morphotype | Siphoviridae |
| Number of Genes | 240 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF006818 |
| Refseq Number | NC_022067 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
22 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |