| Discovery Information |
| Isolation Host | Rhodococcus erythropolis NRRL B-1574 |
| Former names | Camophlage |
| Found By | Ezekiel Robinson, Matthew Cox |
| Year Found | 2018 |
| Location Found | Orono, ME USA |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.897511 N, 68.668791 W Map |
| Discovery Notes | Temp: 55 degrees Fahrenheit
Location: University of Maine Orono campus. Soil sample was taken from a decorative garden planted against the side of the green house |
| Naming Notes | We mistook an agar chunk for our phage, it was camouflaged. Hence, camophlage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 332 |
| Genome length (bp) | 49660 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGGCCGCCCTA |
| GC Content | 61.9% |
| Sequencing Notes | Around 24100 in this sequence, there were some conflicting reads. There's also a 30-ish bp repeat here. This suggests perhaps there is a sub-population that has a different number (2?) of repeats than the final published sequence (3). |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | Small circular plaques (.5 mm)
Small circular plaques (1.0 mm)
slightly turbid |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK967393 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
