Discovery Information |
Isolation Host | Rhodococcus erythropolis NRRL B-1574 |
Former names | Camophlage |
Found By | Ezekiel Robinson, Matthew Cox |
Year Found | 2018 |
Location Found | Orono, ME USA |
Finding Institution | University of Maine, Honors College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 44.897511 N, 68.668791 W Map |
Discovery Notes | Temp: 55 degrees Fahrenheit
Location: University of Maine Orono campus. Soil sample was taken from a decorative garden planted against the side of the green house |
Naming Notes | We mistook an agar chunk for our phage, it was camouflaged. Hence, camophlage. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 15, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 332 |
Genome length (bp) | 49660 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | TGGCCGCCCTA |
GC Content | 61.9% |
Sequencing Notes | Around 24100 in this sequence, there were some conflicting reads. There's also a 30-ish bp repeat here. This suggests perhaps there is a sub-population that has a different number (2?) of repeats than the final published sequence (3). |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | Singleton |
Subcluster | -- |
Cluster Life Cycle | Unknown |
Other Cluster Members |
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Annotating Institution | University of Maine, Honors College |
Annotation Status | In GenBank |
Plaque Notes | Small circular plaques (.5 mm) Small circular plaques (1.0 mm) slightly turbid |
Number of Genes | 77 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK967393 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
85 |
Pitt Freezer Box Grid# |
A10 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |