| Detailed Information for Phage Zagie |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alyssa Haworth and Emma Hanson |
| Year Found | 2017 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.667367 N, 117.402273 W Map |
| Discovery Notes | We decided to use our results from our second sample: Sample 2: surfaced grass not shaded (dry, warm) Northeast of the Hughes building and on the Herak Quad. |
| Naming Notes | Our phage's name is Zagie, which is derived from the name Magie/Maggie and it is a reference to the term "Zag" since we attend Gonzaga University. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 19, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 342 |
| Genome length (bp) | 62442 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 204 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | After performing our first attempt to begin forming phage-- using serial dilutions and plaque assay-- we received our first results:
We found phage within our petri dishes of 10^0, 10^-1, and one in 10^-2. Our 10^0 had phage only on half of the petri dish. Could it be because the dish was tilted to one side due to the table being slanted? The plate colors were a turbid yellow. 10^-1 had approximately 30 phage and was too tilted to one side. 10^-2 only had 2 phage, and after 10^-3, they were all solid with no sign of phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP068344 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
65 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
