| Detailed Information for Phage Cota | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Rocky Ng |
| Year Found | 2013 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.068582 N, 118.441285 W Map |
| Discovery Notes | Team Breakphage Club (Stephen Wandro, Rocky Ng, Cynthia Mund and Fernando Morales) isolated and characterized phage Cota from a pore sample. TA: Samantha Capati, Instructor: Jordan Moberg Parker Annotation was done by MIMG 103AL-F15 class Instructor: Giorgia Pirino, Additional annotation by Rocky Ng and Jordan Moberg Parker. Collection Date: October 4, 2013 Sample type: Biore pore strip Sample description: Nose Host range analysis: Able to infect laboratory P. acnes, putative P. acnes from Stephen, Cynthia, and Natalie |
| Naming Notes | Pronounced "Caught-Ah"; the team was very excited when they finally caught a phage, so Cota Phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 11, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 11129 |
| Genome length (bp) | 29549 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.0% |
| Sequencing Notes | There is a small SNP in the population. Approximately 20% of the reads had a C instead of an A at position 14183. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Bullseye morphology at higher titers. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Higher titers have more turbid plaques, lower titers have clear plaques. Clear; 6.3mm average. |
| Morphotype | Siphoviridae |
| Number of Genes | 48 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewCota_1 Cota_2 Cota_3 Cota_4 Cota_5 Cota_6 Cota_7 Cota_8 Cota_9 Cota_10 Cota_11 Cota_12 Cota_13 Cota_14 Cota_15 Cota_16 Cota_17 Cota_18 Cota_19 Cota_20 Cota_21 Cota_22 Cota_23 Cota_24 Cota_25 Cota_26 Cota_27 Cota_28 Cota_29 Cota_30 Cota_31 Cota_32 Cota_33 Cota_34 Cota_35 Cota_36 Cota_37 Cota_38 Cota_39 Cota_40 Cota_41 Cota_42 Cota_43 Cota_44 Cota_45 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN329678 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | B12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |