| Detailed Information for Phage Sekhmet |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | Pamuda |
| Found By | Brenden Johnson |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.442391 N, 79.952848 W Map |
| Discovery Notes | When the sample yielding this phage was collected (9:52 AM on 9-12-18), the temperature was approximately 65 degrees Fahrenheit and the weather was cloudy. The soil was dark, indicating nutrient-richness, and the area surrounding the collection location was a commonly walked pathway with plenty of detritus but little vegetation. |
| Naming Notes | Sekhmet, in ancient Egyptian religion, was a warrior goddess whose name was often associated with rivalry and change. The rationale behind naming this particular phage Sekhmet is derived from the theory of a constant evolutionary battle between phages and their host bacteria. Just as bacteria have attained uncountable adaptations over their billions of years of coexistence with bacteriophages to prevent and deal with infection, phages have also adapted to more efficiently infect and propagate using their hosts. In this way, the population of bacteriophages is a symbol in biology much like Sekhmet was to the Egyptian people; wagers of war who trigger massive change in the world. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 8, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 501 |
| Genome length (bp) | 47735 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are irregular but clear ranging from 0.5 and 3 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937609 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
10^10 pfu/mL |
| Pitt Freezer Box# |
91 |
| Pitt Freezer Box Grid# |
B4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
