Gordonia phage Cynthia
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Detailed Information for Phage Cynthia
Discovery Information
Isolation HostGordonia terrae 3612
Found ByHaley Shuford
Year Found2017
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.445278 N, 79.961111 W Map
Discovery NotesFound at 36 degrees Fahrenheit on 1/26/17 at 12:59 PM. Environmental conditions were mostly cloudy skies with recent precipitation and snow flurries. Collected from soil next to a sidewalk in front of a dormitory building. Soil collection was damp, cold, mud consistency, and medium brown color. Approximately half a cup was obtained. Collected in a plastic bag. Transported for 3 minutes and stored on the surface of a desk at 72.5 degrees Fahrenheit for one and a half hours.
Naming NotesName came from a popular movie.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 14, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1206
Genome length (bp)46219
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Other Cluster Members
Plaque NotesPlaques of Phage Cynthia are of two different sizes. One larger plaque, approximately one nanometer in size, and one smaller plaque, approximately the size of a pin prick. Both plaque sizes are of clear nature and are circular in shape.
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload