Microbacterium phage BabyDaisy
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Detailed Information for Phage BabyDaisy
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByTaylor Hart
Year Found2021
Location FoundPeabody, MA United States
Finding InstitutionSalem State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates42.544262 N, 71.007661 W Map
Discovery NotesRich garden soil taken 22cm beneath a compost garden on September 20th, 2021. The soil was moist and contained leaves, roots, and pearlite.
Naming NotesThis phage was named after my infant daughter, Daisy Jean Stuart.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1433
Genome length (bp)42226
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceACTCCCGACA
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionSalem State University
Annotation StatusIn GenBank
Plaque NotesSmall, pinprick sized clear plaques.
Number of Genes71
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

BabyDaisy_1

BabyDaisy_2

BabyDaisy_3
(terminase small subunit)

BabyDaisy_4
(terminase large subunit)

BabyDaisy_5
(portal protein)

BabyDaisy_6
(capsid maturation protease)

BabyDaisy_7
(major capsid protein)

BabyDaisy_8

BabyDaisy_9
(head-to-tail adaptor)

BabyDaisy_10
(head-to-tail stopper)

BabyDaisy_11

BabyDaisy_12
(tail terminator)

BabyDaisy_13
(major tail protein)

BabyDaisy_14
(tail assembly chaperone)

BabyDaisy_15
(tail assembly chaperone)

BabyDaisy_16

BabyDaisy_17
(tape measure protein)

BabyDaisy_18
(minor tail protein)

BabyDaisy_19
(minor tail protein)

BabyDaisy_20

BabyDaisy_21
(minor tail protein)

BabyDaisy_22
(minor tail protein)

BabyDaisy_23
(endolysin)

BabyDaisy_24
(holin)

BabyDaisy_25

BabyDaisy_26

BabyDaisy_27

BabyDaisy_28

BabyDaisy_29
(Cas4 exonuclease)

BabyDaisy_30

BabyDaisy_31
(deoxyuridine triphosphatase)

BabyDaisy_32
(thymidylate kinase)

BabyDaisy_33
(recombination directionality factor)

BabyDaisy_34

BabyDaisy_35
(NrdH-like glutaredoxin)

BabyDaisy_36
(Holliday junction resolvase)

BabyDaisy_37

BabyDaisy_38

BabyDaisy_39

BabyDaisy_40

BabyDaisy_41
(DNA primase/helicase)

BabyDaisy_42
(DNA polymerase I)

BabyDaisy_43

BabyDaisy_44

BabyDaisy_45
(DNA binding protein)

BabyDaisy_46

BabyDaisy_47

BabyDaisy_48

BabyDaisy_49
(ParB-like nuclease domain protein)

BabyDaisy_50

BabyDaisy_51

BabyDaisy_52
(HNH endonuclease)

BabyDaisy_53

BabyDaisy_54

BabyDaisy_55

BabyDaisy_56

BabyDaisy_57

BabyDaisy_58
(dihydrofolate reductase)

BabyDaisy_59

BabyDaisy_60

BabyDaisy_61
(thymidylate synthase)

BabyDaisy_62

BabyDaisy_63

BabyDaisy_64

BabyDaisy_65

BabyDaisy_66

BabyDaisy_67

BabyDaisy_68

BabyDaisy_69

BabyDaisy_70 — tRNA
(tRNA-Gln(ctg))

BabyDaisy_71
(HNH endonuclease)

BabyDaisy_72

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR521064
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload