| Detailed Information for Phage OneinaGillian |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gillian |
| Year Found | 2018 |
| Location Found | Riverside, CA USA |
| Finding Institution | La Sierra University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.912528 N, 117.500972 W Map |
| Discovery Notes | -collected under the tree next to the back door of Angwin Hall.
-had a lot of organic matter like worms, grass, and twigs.
-sample was taken on January 9th at 5:53p.m.
-soil was wet due to rain.
-about 63 degrees Fahrenheit outside |
| Naming Notes | Since my name is Gillian Miller, I wanted to use my name as a play-on word. A gillion means, in a literal sense, a thousand billion, but it can also mean rare or ambiguous. The meaning behind my names is that this phage may seem insignificant because there are so many phages in the world, but it is special because it is different and one out of the 10^31 phages on the earth. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 26, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2049 |
| Genome length (bp) | 61703 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 203 bp |
| End Determination Notes | The right end of the terminal repeat was clear from a large buildup of reads. The left end of the terminal repeat was less clear, and had to be determined through similarity to other genomes and the drop in double coverage. |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | La Sierra University |
| Annotation Status | In GenBank |
| Plaque Notes | -Cloudy, small circles |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727556 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
