| Detailed Information for Phage PastaFagioli | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Chris Kachi |
| Year Found | 2024 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 23°C |
| GPS Coordinates | 45.38661 N, 75.68198 W Map |
| Discovery Notes | This phage was discovered in a bog near the Ottawa Rideau River. |
| Naming Notes | The name for this phage comes from an Italian dish that I grew up eating and continue to eat regularly. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2055 |
| Genome length (bp) | 42242 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGACA |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAbigail Akino08 Albedo Albright AnnaLie Armstrong Arroyo AvGardian Avocadoman BabyDaisy BabyYoda Bachaco BAjuniper BelmontSKP Bengal Bernstein Brahms BubbaBear Buldak Burritobowl Cashington Celaena ChiliPepper Clayda5 CliffHanger Coltrane CroZenni CupcakePrincess DickRichards Didgeridoo DirtyBubble Dismas Doobus Eden Elva Eula Finalfrontier FlameThrower Franklin22 Gack Icarian IndyLu Jabb Johnathan Jovita Kate33 Katzastrophic Kenzers Kieran Lahqtemish LimaBean Loviatar Lynlen Milomuff MsUbiquitous Nicky22 Olliecat PastaFagioli PhigPhack Phisb QMacho Quenya Rollins Rona SanaSana SansAfet SarBear Sharkboy Shayna Skylord Slay Softsoap Solea Squircle Stoor Stromboli SunnyD Swervy Teagster TukTuk Vitas WalkingDead |
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | The plaques of PastaFagioli are clear, small, and circular. The clear appearance indicates effective bacterial lysis, while the small size suggests a narrow host range or moderate replication rate. The circular shape reflects uniform lysis across the bacterial lawn, typical of phages with consistent infection patterns. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPastaFagioli_1 PastaFagioli_2 PastaFagioli_3 PastaFagioli_4 PastaFagioli_5 PastaFagioli_6 PastaFagioli_7 PastaFagioli_8 PastaFagioli_9 PastaFagioli_10 PastaFagioli_11 PastaFagioli_12 PastaFagioli_13 PastaFagioli_14 PastaFagioli_15 PastaFagioli_16 PastaFagioli_17 PastaFagioli_18 PastaFagioli_19 PastaFagioli_20 PastaFagioli_21 PastaFagioli_22 PastaFagioli_23 PastaFagioli_24 PastaFagioli_25 PastaFagioli_26 PastaFagioli_27 PastaFagioli_28 PastaFagioli_29 PastaFagioli_30 PastaFagioli_31 PastaFagioli_32 PastaFagioli_33 PastaFagioli_34 PastaFagioli_35 PastaFagioli_36 PastaFagioli_37 PastaFagioli_38 PastaFagioli_39 PastaFagioli_40 PastaFagioli_41 PastaFagioli_42 PastaFagioli_43 PastaFagioli_44 PastaFagioli_45 PastaFagioli_46 PastaFagioli_47 PastaFagioli_48 PastaFagioli_49 PastaFagioli_50 PastaFagioli_51 PastaFagioli_52 PastaFagioli_53 PastaFagioli_54 PastaFagioli_55 PastaFagioli_56 PastaFagioli_57 PastaFagioli_58 PastaFagioli_59 PastaFagioli_60 PastaFagioli_61 PastaFagioli_62 PastaFagioli_63 PastaFagioli_64 PastaFagioli_65 PastaFagioli_66 PastaFagioli_67 PastaFagioli_68 — tRNA PastaFagioli_69 PastaFagioli_70 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
