| Detailed Information for Phage PastaFagioli |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Chris Kachi |
| Year Found | 2024 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 23°C |
| GPS Coordinates | 45.38661 N, 75.68198 W Map |
| Discovery Notes | This phage was discovered in a bog near the Ottawa Rideau River. |
| Naming Notes | The name for this phage comes from an Italian dish that I grew up eating and continue to eat regularly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2055 |
| Genome length (bp) | 42242 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGACA |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | QC Complete |
| Plaque Notes | The plaques of PastaFagioli are clear, small, and circular. The clear appearance indicates effective bacterial lysis, while the small size suggests a narrow host range or moderate replication rate. The circular shape reflects uniform lysis across the bacterial lawn, typical of phages with consistent infection patterns. |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
203 |
| Pitt Freezer Box Grid# |
D4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
