| Detailed Information for Phage Zhafia |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kira Bifone, Giselle Tevlin |
| Year Found | 2022 |
| Location Found | Spokane, WA United States |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.668573 N, 117.403505 W Map |
| Discovery Notes | On August 30, 2022, when the soil sample was collected the soil was slightly damp. It was easy to scoop and found surrounded by grass. The weather was warm. |
| Naming Notes | Combination of two friends' names. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 6, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 112 |
| Genome length (bp) | 62287 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 199 bp |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques from the phage Zhafia are small and cloudy around the edges. They range in size from 1 mm to 5mm when grouped in close proximity. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PX307258 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
166 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
