Discovery Information |
Isolation Host | Microbacterium foliorum NRRL B-24224 |
Found By | Stephanie García Hernández |
Year Found | 2022 |
Location Found | Aguas Buenas, PR Puerto Rico |
Finding Institution | Universidad Ana G. Mendez, Gurabo campus |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 83°C |
GPS Coordinates | 18.29045 N, 66.07176 W Map |
Discovery Notes | The sample was taken from a pot where a flowering plant is located. The soil was fertile and moist, had a fertilizer of several months for the plant. The soil had different plants around it so when taking the sample there were also leaves, roots, also ants and fungi. |
Naming Notes | The name chosen is due to the fact that when we worked the dilutions in series the plaques of my phage were not appreciated quickly, it took approximately two (2) days for the plaques to be visible, so it seemed like the phage "played" hide and seek in the bacteria. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 19, 2023 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2275 |
Genome length (bp) | 41843 |
Character of genome ends | Circularly Permuted |
GC Content | 63.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | EA |
Subcluster | EA1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
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Annotating Institution | Universidad Ana G. Mendez, Gurabo campus |
Annotation Status | In GenBank |
Plaque Notes | The plaques were not very well appreciated without light so with the help of a backlight. They are cloudy and small plaques. |
Number of Genes | 64 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | PP978889 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Not in Pitt Archives |
Available Files |
Plaque Picture | Download |
GenBank File for Phamerator | Download |