Microbacterium phage Winzigespinne
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Detailed Information for Phage Winzigespinne
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByAdam Saal
Year Found2019
Location FoundOttawa, Canada
Finding InstitutionUniversity of Ottawa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature15°C
GPS Coordinates45.4242 N, 75.6829 W Map
Discovery NotesPhage environmental sample collected from a local garden. Unknown if garden was fertilized.
Soil was soft and airy
Soil was dark in colour
Naming NotesWinzigespinne is roughly translated "Tiny Spider" to German. The inspiration comes from a reference mentioned in Stephanie Strathdee's novel "The Perfect Predator". It references the nursery rhyme "There was an old lady who swallowed a fly". The old lady swallows a spider to catch the fly. Strathdee compares the fly to an infectious bacteria and the phage the spider to catch that fly.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1592
Genome length (bp)41556
Character of genome endsCircularly Permuted
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Other Cluster Members

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Mercedes (None)

AlexAdler (EA1)

Alyxandracam (EA1)

Antoinette (EA1)

Aubergine (EA1)

AxiPup (EA1)

Baines (EA1)

Balsa (EA1)

Bandik (EA1)

BeautPeep30 (EA1)

BeeBee8 (EA1)

Benjalauren (EA1)

BigRedClifford (EA1)

BonesMcCoy (EA1)

Bonino (EA1)

Calix (EA1)

Chamuel (EA1)

Christoph (EA1)

Clancy (EA1)

Convict (EA1)

Dave (EA1)

Den3 (EA1)

Dothraki (EA1)

Erla (EA1)

Espinosa (EA1)

Etna (EA1)

Etta (EA1)

Gargoyle (EA1)

Gelo (EA1)

Gershwin (EA1)

Greys (EA1)

Gubbabump (EA1)

Hamlet (EA1)

HanSolo (EA1)

Ilzat (EA1)

Ioannes (EA1)

Jenos (EA1)

Kale (EA1)

Klimt (EA1)

Knox (EA1)

Kurt1 (EA1)

Leafus (EA1)

Lovelyunicorn (EA1)

Ludgate (EA1)

MaeLinda (EA1)

ManRay (EA1)

Martin (EA1)

McGalleon (EA1)

MillyPhilly (EA1)

MonChoix (EA1)

MrWorldwide (EA1)

Nagem (EA1)

Nattles (EA1)

Oats (EA1)

Oxtober96 (EA1)

Papafritta (EA1)

ParleG (EA1)

Peep (EA1)

Peppino (EA1)

Pherferi (EA1)

Phriends (EA1)

Pocket (EA1)

PuppyEggo (EA1)

Raccoon (EA1)

Raptor (EA1)

Redfield (EA1)

Renzie (EA1)

Riyhil (EA1)

Robinson (EA1)

Schnapsidee (EA1)

Sedgewig (EA1)

Shee (EA1)

SonOfLevi (EA1)

Stanktossa (EA1)

StingRay (EA1)

Strathdee (EA1)

Superfresh (EA1)

TatarkaPM (EA1)

Teagan (EA1)

TeddyBear (EA1)

Tenda (EA1)

Thorongil (EA1)

TinSulphur (EA1)

Velene (EA1)

WildNOut (EA1)

Winzigespinne (EA1)

Zada (EA1)

Nucci (EA10)

Quartz (EA10)

YuuY (EA10)

Ixel (EA11)

Andromedas (EA2)

ColaCorta (EA2)

Eleri (EA2)

Finny (EA2)

Glamour (EA2)

MCubed (EA2)

Sansa (EA2)

Casey (EA3)

Pajaza (EA3)

Pikmin (EA3)

Bustleton (EA4)

Golden (EA4)

Kauala (EA4)

Koji (EA4)

Lucky3 (EA4)

Pherbot (EA4)

PrincePhergus (EA4)

Sinatra (EA4)

Alakazam (EA5)

Nebulous (EA5)

Neferthena (EA5)

QuadZero (EA5)

Zayuliv (EA5)

Zepp (EA5)

Chepli (EA6)

WilliamStrong (EA6)

Theresita (EA7)

Schubert (EA8)

ChickenKing (EA9)

GaeCeo (EA9)

Annotating InstitutionUniversity of Ottawa
Annotation StatusIn GenBank
Plaque NotesPlaques are partialy turbid. Small to medium in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Winzigespinne_1

Winzigespinne_2
(terminase)

Winzigespinne_3
(portal protein)

Winzigespinne_4
(MuF-like minor capsid protein)

Winzigespinne_5
(scaffolding protein)

Winzigespinne_6
(major capsid protein)

Winzigespinne_7

Winzigespinne_8

Winzigespinne_9

Winzigespinne_10

Winzigespinne_11

Winzigespinne_12
(tail terminator)

Winzigespinne_13

Winzigespinne_14
(major tail protein)

Winzigespinne_15
(tail assembly chaperone)

Winzigespinne_16
(tail assembly chaperone)

Winzigespinne_17
(tape measure protein)

Winzigespinne_18
(minor tail protein)

Winzigespinne_19
(minor tail protein)

Winzigespinne_20

Winzigespinne_21

Winzigespinne_22

Winzigespinne_23
(minor tail protein)

Winzigespinne_24
(lysin A)

Winzigespinne_25

Winzigespinne_26
(holin)

Winzigespinne_27

Winzigespinne_28

Winzigespinne_29

Winzigespinne_30

Winzigespinne_31

Winzigespinne_32

Winzigespinne_33
(RecA-like DNA recombinase)

Winzigespinne_34
(nuclease)

Winzigespinne_35

Winzigespinne_36
(AAA-ATPase)

Winzigespinne_37
(exonuclease)

Winzigespinne_38
(DNA polymerase I)

Winzigespinne_39

Winzigespinne_40
(DNA helicase)

Winzigespinne_41
(phosphoesterase)

Winzigespinne_42

Winzigespinne_43

Winzigespinne_44
(MazG-like nucleotide pyrophosphohydrolase)

Winzigespinne_45

Winzigespinne_46
(AAA-ATPase)

Winzigespinne_47
(glycosyltransferase)

Winzigespinne_48

Winzigespinne_49

Winzigespinne_50
(thymidylate synthase)

Winzigespinne_51

Winzigespinne_52

Winzigespinne_53

Winzigespinne_54

Winzigespinne_55

Winzigespinne_56

Winzigespinne_57

Winzigespinne_58

Winzigespinne_59

Winzigespinne_60

Winzigespinne_61

Winzigespinne_62

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT986027
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 106
Pitt Freezer Box Grid# E6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload