The Actinobacteriophage Database

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Detailed Information for Phage Librie
Discovery Information
Isolation Host	Microbacterium foliorum NRRL B-24224
Found By	Skyelar A. Brooks
Year Found	2021
Location Found	Clarksville, TN USA
Finding Institution	Austin Peay State University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	10°C
GPS Coordinates	36.531077 N, 87.357418 W Map
Discovery Notes	Soil was from an outdoor garden area. Will S. Primasing, David M. Hale, Joseph A. Vogelpohl and Mia Gracia-Toro helped to purify this phage and to isolate its DNA
Naming Notes	The phage was found near the Austin Peay State University bookstore. In Latin, liber mean book.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 12, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	20
Genome length (bp)	39941
Character of genome ends	Circularly Permuted
GC Content	63.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	EA
Subcluster	EA5
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Mercedes (None) Acosta (EA1) Agente005 (EA1) AlexAdler (EA1) Alyxandracam (EA1) Anilorac (EA1) Antoinette (EA1) AranulaLuti (EA1) Asta (EA1) Aubergine (EA1) AxiPup (EA1) Baines (EA1) Balsa (EA1) Bandik (EA1) BeautPeep30 (EA1) BeeBee8 (EA1) Benjalauren (EA1) BigRedClifford (EA1) Blage (EA1) BonesMcCoy (EA1) Bonino (EA1) Byron23 (EA1) Calix (EA1) CarisSwetlik (EA1) Chako (EA1) Chamuel (EA1) Christoph (EA1) Clancy (EA1) Convict (EA1) Dave (EA1) Delphidian (EA1) Den3 (EA1) Dothraki (EA1) Duocatuli (EA1) Endor (EA1) Erla (EA1) Espinosa (EA1) Etna (EA1) Etta (EA1) Exploradora (EA1) FarmerDoug (EA1) Figueroism (EA1) GardenB (EA1) Garey24 (EA1) Gargoyle (EA1) Gelo (EA1) Gershwin (EA1) Greys (EA1) Gubbabump (EA1) Hamlet (EA1) HankSprout (EA1) HanSolo (EA1) HungryHenry (EA1) Ilzat (EA1) Inventa (EA1) Ioannes (EA1) Janus167 (EA1) JasonD (EA1) JasperRussell (EA1) Jenos (EA1) Jerbirus (EA1) JeriBeth (EA1) Kale (EA1) KannH (EA1) KingJulien (EA1) Klimt (EA1) Knox (EA1) Kurt1 (EA1) Leafus (EA1) Lovelyunicorn (EA1) Ludgate (EA1) MaeLinda (EA1) ManRay (EA1) Martin (EA1) McGalleon (EA1) MillyPhilly (EA1) MonChoix (EA1) MrWorldwide (EA1) Nagem (EA1) Nattles (EA1) NickSell (EA1) Oats (EA1) OldNelly (EA1) Oxtober96 (EA1) Papafritta (EA1) ParleG (EA1) Peep (EA1) Peppino (EA1) Pherferi (EA1) Phiderman (EA1) Phireproof (EA1) PhredFlintston (EA1) Phriends (EA1) Pocket (EA1) PuppyEggo (EA1) Raccoon (EA1) Rapheph (EA1) Raptor (EA1) Redfield (EA1) Renzie (EA1) Riyhil (EA1) Robinson (EA1) Rog141 (EA1) RosieMae (EA1) Schnapsidee (EA1) Sedgewig (EA1) ShaiHulud (EA1) Shee (EA1) SJay (EA1) SoilGremlin (EA1) SonOfLevi (EA1) Stanktossa (EA1) StingRay (EA1) StirfryIV (EA1) Stormbreaker8 (EA1) Strathdee (EA1) Superfresh (EA1) Symere (EA1) TatarkaPM (EA1) Teagan (EA1) TeddyBear (EA1) Tenda (EA1) Thompsone (EA1) Thorongil (EA1) TinSulphur (EA1) Velene (EA1) Vispistious (EA1) WildNOut (EA1) Winzigespinne (EA1) Zada (EA1) Carostasia (EA10) HairyMan (EA10) Juanyo (EA10) Mandalorian (EA10) Nucci (EA10) Quartz (EA10) YuuY (EA10) HerculesXL (EA11) Ixel (EA11) Schimmels22 (EA11) Scissor2024 (EA11) Tinyman4 (EA11) WestPM (EA11) Andromedas (EA2) ChikPic (EA2) ColaCorta (EA2) Eleri (EA2) Finny (EA2) Glamour (EA2) MCubed (EA2) Phanita (EA2) PSirce (EA2) Sansa (EA2) Saratos (EA2) Shamu (EA2) Zenitsu (EA2) Casey (EA3) Pajaza (EA3) Pikmin (EA3) BouleyBill (EA4) Bustleton (EA4) Golden (EA4) Guetzie (EA4) Jingles (EA4) Kauala (EA4) Koji (EA4) Lucky3 (EA4) Pherbot (EA4) PrincePhergus (EA4) RenegadeRaider (EA4) Sinatra (EA4) SirVictor (EA4) Alakazam (EA5) BaronJohn (EA5) CaptainRex (EA5) Clock (EA5) Fulton (EA5) GreenIvy (EA5) Hasitha (EA5) Librie (EA5) LilTerminator (EA5) Nebulous (EA5) Neferthena (EA5) QuadZero (EA5) TwoBits (EA5) Wardwill (EA5) Zayuliv (EA5) Zepp (EA5) Chepli (EA6) Jemerald (EA6) Juicer (EA6) KatChan (EA6) Luna18 (EA6) Morrigan (EA6) WilliamStrong (EA6) Theresita (EA7) Schubert (EA8) Cheeto1 (EA9) ChickenKing (EA9) GaeCeo (EA9)
Annotating Institution	Austin Peay State University
Annotation Status	In GenBank
Plaque Notes	Medium size plaques
Morphotype	Siphoviridae
Number of Genes	61
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Librie_1 Librie_2 (terminase) Librie_3 Librie_4 (portal protein) Librie_5 Librie_6 Librie_7 (scaffolding protein) Librie_8 (major capsid protein) Librie_9 Librie_10 Librie_11 Librie_12 Librie_13 (tail terminator) Librie_14 Librie_15 (major tail protein) Librie_16 (tail assembly chaperone) Librie_17 (tail assembly chaperone) Librie_18 (tape measure protein) Librie_19 (minor tail protein) Librie_20 (minor tail protein) Librie_21 (minor tail protein) Librie_22 Librie_23 (endolysin) Librie_24 Librie_25 Librie_26 Librie_27 Librie_28 Librie_29 Librie_30 Librie_31 (DNA primase/helicase) Librie_32 (nuclease) Librie_33 Librie_34 (AAA-ATPase) Librie_35 (Cas4 family exonuclease) Librie_36 Librie_37 (DNA polymerase I) Librie_38 — tRNA (tRNA-Undet(nnn)) Librie_39 Librie_40 (DNA helicase) Librie_41 Librie_42 Librie_43 (MazG-like nucleotide pyrophosphohydrolase) Librie_44 Librie_45 (thymidylate kinase) Librie_46 (glycosyltransferase) Librie_47 (glycosyltransferase) Librie_48 Librie_49 Librie_50 (thymidylate synthase) Librie_51 Librie_52 Librie_53 Librie_54 Librie_55 Librie_56 Librie_57 Librie_58 Librie_59 Librie_60 Librie_61 Librie_62
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	ON970570
Refseq Number	None yet
Published in Paper	Markov et al 2022, Microbiology Resource Announcements: Complete Genome Sequence of Microbacterium foliorum Bacteriophage Librie
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	136
Pitt Freezer Box Grid#	F1
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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