Microbacterium phage Rapheph
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Detailed Information for Phage Rapheph
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByRafef Elsaid, Ariannah Lowe, and Juliana Buccieri
Year Found2022
Location FoundUpper Darby Township, PA United States of America
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature23°C
GPS Coordinates39.96393 N, 75.27934 W Map
Discovery NotesThe soil was dug on September 20th, 2022 at 9:27 pm. The sample was collected from the base of a plant in the shade, in samp soil that was dug 2-3 inches deep. the general location was Upper Darby Philadelphia.

the soil collection, direct and enriched isolation, initial plaque assay, and spot test was completed by Rafef Elsaid.
The phage was then adopted by lab partners, Juliana Buccieri and Ariannah Lowe who began working with the phage at the purification stage.
Naming NotesJuliana and Ariannah named their given phage Rapheph after the student, Rafef Elsaid who so kindly donated their phage so that the pair could continue their research.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1939
Genome length (bp)41805
Character of genome endsCircularly Permuted
GC Content63.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Other Cluster Members

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Mercedes (None)

Acosta (EA1)

Agente005 (EA1)

AlexAdler (EA1)

Alyxandracam (EA1)

Antoinette (EA1)

AranulaLuti (EA1)

Asta (EA1)

Aubergine (EA1)

AxiPup (EA1)

Baines (EA1)

Balsa (EA1)

Bandik (EA1)

BeautPeep30 (EA1)

BeeBee8 (EA1)

Benjalauren (EA1)

BigRedClifford (EA1)

Blage (EA1)

BonesMcCoy (EA1)

Bonino (EA1)

Byron23 (EA1)

Calix (EA1)

Chako (EA1)

Chamuel (EA1)

Christoph (EA1)

Clancy (EA1)

Convict (EA1)

Dave (EA1)

Delphidian (EA1)

Den3 (EA1)

Dothraki (EA1)

Duocatuli (EA1)

Endor (EA1)

Erla (EA1)

Espinosa (EA1)

Etna (EA1)

Etta (EA1)

FarmerDoug (EA1)

Figueroism (EA1)

GardenB (EA1)

Garey24 (EA1)

Gargoyle (EA1)

Gelo (EA1)

Gershwin (EA1)

Greys (EA1)

Gubbabump (EA1)

Hamlet (EA1)

HankSprout (EA1)

HanSolo (EA1)

HungryHenry (EA1)

Ilzat (EA1)

Inventa (EA1)

Ioannes (EA1)

Janus167 (EA1)

JasonD (EA1)

JasperRussell (EA1)

Jenos (EA1)

Jerbirus (EA1)

JeriBeth (EA1)

Kale (EA1)

KannH (EA1)

KingJulien (EA1)

Klimt (EA1)

Knox (EA1)

Kurt1 (EA1)

Leafus (EA1)

Lovelyunicorn (EA1)

Ludgate (EA1)

MaeLinda (EA1)

ManRay (EA1)

Martin (EA1)

McGalleon (EA1)

MillyPhilly (EA1)

MonChoix (EA1)

MrWorldwide (EA1)

Nagem (EA1)

Nattles (EA1)

NickSell (EA1)

Oats (EA1)

OldNelly (EA1)

Oxtober96 (EA1)

Papafritta (EA1)

ParleG (EA1)

Peep (EA1)

Peppino (EA1)

Pherferi (EA1)

Phiderman (EA1)

Phireproof (EA1)

PhredFlintston (EA1)

Phriends (EA1)

Pocket (EA1)

PuppyEggo (EA1)

Raccoon (EA1)

Rapheph (EA1)

Raptor (EA1)

Redfield (EA1)

Renzie (EA1)

Riyhil (EA1)

Robinson (EA1)

Rog141 (EA1)

Schnapsidee (EA1)

Sedgewig (EA1)

ShaiHulud (EA1)

Shee (EA1)

SJay (EA1)

SoilGremlin (EA1)

SonOfLevi (EA1)

Stanktossa (EA1)

StingRay (EA1)

StirfryIV (EA1)

Stormbreaker8 (EA1)

Strathdee (EA1)

Superfresh (EA1)

TatarkaPM (EA1)

Teagan (EA1)

TeddyBear (EA1)

Tenda (EA1)

Thompsone (EA1)

Thorongil (EA1)

TinSulphur (EA1)

Velene (EA1)

Vispistious (EA1)

WildNOut (EA1)

Winzigespinne (EA1)

Zada (EA1)

Carostasia (EA10)

Juanyo (EA10)

Mandalorian (EA10)

Nucci (EA10)

Quartz (EA10)

YuuY (EA10)

HerculesXL (EA11)

Ixel (EA11)

Schimmels22 (EA11)

Tinyman4 (EA11)

WestPM (EA11)

Andromedas (EA2)

ChikPic (EA2)

ColaCorta (EA2)

Eleri (EA2)

Finny (EA2)

Glamour (EA2)

MCubed (EA2)

Sansa (EA2)

Saratos (EA2)

Shamu (EA2)

Zenitsu (EA2)

Casey (EA3)

Pajaza (EA3)

Pikmin (EA3)

BouleyBill (EA4)

Bustleton (EA4)

Golden (EA4)

Guetzie (EA4)

Kauala (EA4)

Koji (EA4)

Lucky3 (EA4)

Pherbot (EA4)

PrincePhergus (EA4)

Sinatra (EA4)

SirVictor (EA4)

Alakazam (EA5)

CaptainRex (EA5)

Clock (EA5)

Fulton (EA5)

GreenIvy (EA5)

Hasitha (EA5)

Librie (EA5)

LilTerminator (EA5)

Nebulous (EA5)

Neferthena (EA5)

QuadZero (EA5)

Zayuliv (EA5)

Zepp (EA5)

Chepli (EA6)

Jemerald (EA6)

Juicer (EA6)

KatChan (EA6)

Luna18 (EA6)

Morrigan (EA6)

WilliamStrong (EA6)

Theresita (EA7)

Schubert (EA8)

Cheeto1 (EA9)

ChickenKing (EA9)

GaeCeo (EA9)

Annotating InstitutionDrexel University
Annotation StatusIn GenBank
Plaque NotesThe plaque we purified had bullseyes throughout and was cloudy on the edges but clear in the center. The plaques were not a definite circle shape but rather rough around the edges.
Number of Genes63
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR613481
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 161
Pitt Freezer Box Grid# H10
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload