Microbacterium phage BeautPeep30
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Detailed Information for Phage BeautPeep30
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Former namesPhruitLoop
Found BySydni Ernst, Joseph Rajasekaran
Year Found2019
Location FoundPhiladelphia, PA United States of America
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates39.953 N, 75.186 W Map
Discovery NotesDry soil collected from under mulch, a few feet away from a short, young tree. Many weak roots were avoided when collecting the sample. General Vicinity: under blue Drexel University Bridge.
Naming NotesThe phage PhruitLoop came from the cat Fruit Lööp. Since umlauts and other special characters cannot be used, the name PhruitLoop was used instead. The "Ph" is a pun on the word "phage."

The name PhruitLoop was vetoed as it was a homophone of the phage named FruitLoop.

The name BeautPeep30 originates from the song "Beautiful People" by Ed Sheeran. The number 30 was chosen because it was found on 30th street in Philadelphia.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 1, 2019
Approximate Shotgun Coverage1823
Genome length (bp)41809
Character of genome endsCircularly Permuted
GC Content63.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Other Cluster Members

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Mercedes (None)

AlexAdler (EA1)

Alyxandracam (EA1)

Antoinette (EA1)

Aubergine (EA1)

AxiPup (EA1)

Baines (EA1)

Balsa (EA1)

Bandik (EA1)

BeautPeep30 (EA1)

BeeBee8 (EA1)

Benjalauren (EA1)

BigRedClifford (EA1)

BonesMcCoy (EA1)

Bonino (EA1)

Calix (EA1)

Chamuel (EA1)

Christoph (EA1)

Clancy (EA1)

Convict (EA1)

Dave (EA1)

Den3 (EA1)

Dothraki (EA1)

Espinosa (EA1)

Etna (EA1)

Etta (EA1)

Gargoyle (EA1)

Gelo (EA1)

Gershwin (EA1)

Greys (EA1)

Gubbabump (EA1)

Hamlet (EA1)

HanSolo (EA1)

Ilzat (EA1)

Ioannes (EA1)

Jenos (EA1)

Kale (EA1)

Klimt (EA1)

Knox (EA1)

Kurt1 (EA1)

Leafus (EA1)

Lovelyunicorn (EA1)

Ludgate (EA1)

MaeLinda (EA1)

ManRay (EA1)

Martin (EA1)

McGalleon (EA1)

MillyPhilly (EA1)

MonChoix (EA1)

MrWorldwide (EA1)

Nagem (EA1)

Nattles (EA1)

Oats (EA1)

Oxtober96 (EA1)

Papafritta (EA1)

ParleG (EA1)

Peep (EA1)

Peppino (EA1)

Pherferi (EA1)

Phriends (EA1)

Pocket (EA1)

PuppyEggo (EA1)

Raccoon (EA1)

Raptor (EA1)

Redfield (EA1)

Renzie (EA1)

Riyhil (EA1)

Robinson (EA1)

Schnapsidee (EA1)

Sedgewig (EA1)

Shee (EA1)

SonOfLevi (EA1)

Stanktossa (EA1)

StingRay (EA1)

Superfresh (EA1)

TatarkaPM (EA1)

Teagan (EA1)

TeddyBear (EA1)

Tenda (EA1)

Thorongil (EA1)

TinSulphur (EA1)

Velene (EA1)

WildNOut (EA1)

Winzigespinne (EA1)

Zada (EA1)

Nucci (EA10)

Quartz (EA10)

YuuY (EA10)

Ixel (EA11)

Andromedas (EA2)

ColaCorta (EA2)

Eleri (EA2)

Finny (EA2)

Glamour (EA2)

MCubed (EA2)

Sansa (EA2)

Casey (EA3)

Pajaza (EA3)

Pikmin (EA3)

Bustleton (EA4)

Golden (EA4)

Kauala (EA4)

Koji (EA4)

Lucky3 (EA4)

Pherbot (EA4)

PrincePhergus (EA4)

Sinatra (EA4)

Alakazam (EA5)

Nebulous (EA5)

Neferthena (EA5)

QuadZero (EA5)

Zayuliv (EA5)

Zepp (EA5)

Chepli (EA6)

Theresita (EA7)

Schubert (EA8)

ChickenKing (EA9)

GaeCeo (EA9)

Annotating InstitutionDrexel University
Annotation StatusQC Complete
Plaque NotesInitially, the first positive direct isolation of the phage with the M.foliorum host produced a singular clear plaque with sharp edges. Upon purification by serial dilution, more plaques formed with two distinct diameters but otherwise identical morphologies. The larger of the two plaques was ~1.0 cm in diameter and the smaller was ~0.5 cm in diameter. The smaller of the plaques was picked and further purified to produce a pure phage population with consistent diameters of ~0.5cm.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 8.4 x 10^9 pfu/mL
Available Files
GenBank File for PhameratorDownload