Microbacterium phage Duocatuli
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Detailed Information for Phage Duocatuli
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByNatalie Schneider
Year Found2021
Location FoundWashington, MO United States
Finding InstitutionSt. Louis Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates38.546624 N, 91.01274 W Map
Discovery NotesThe sample spot receives morning shade, and afternoon sun. The sample was mostly dry and crumbly. Sample was collected from a six inch depth and stored in a ziplock bag.
Sample was taken at 7:30 am on a cool winters morning (about 40 degrees F) and stored in a refrigerator until I took my hour commute to school at 12:00 pm.
Naming NotesThe phage name is latin for "Two puppies". I discovered Duocatuli from a sample taken from a hole that my two puppies dug in my yard.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 24, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage177
Genome length (bp)41551
Character of genome endsCircularly Permuted
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Other Cluster Members

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Mercedes (None)

AlexAdler (EA1)

Alyxandracam (EA1)

Antoinette (EA1)

AranulaLuti (EA1)

Aubergine (EA1)

AxiPup (EA1)

Baines (EA1)

Balsa (EA1)

Bandik (EA1)

BeautPeep30 (EA1)

BeeBee8 (EA1)

Benjalauren (EA1)

BigRedClifford (EA1)

BonesMcCoy (EA1)

Bonino (EA1)

Calix (EA1)

Chako (EA1)

Chamuel (EA1)

Christoph (EA1)

Clancy (EA1)

Convict (EA1)

Dave (EA1)

Den3 (EA1)

Dothraki (EA1)

Duocatuli (EA1)

Endor (EA1)

Erla (EA1)

Espinosa (EA1)

Etna (EA1)

Etta (EA1)

Figueroism (EA1)

GardenB (EA1)

Gargoyle (EA1)

Gelo (EA1)

Gershwin (EA1)

Greys (EA1)

Gubbabump (EA1)

Hamlet (EA1)

HanSolo (EA1)

Ilzat (EA1)

Inventa (EA1)

Ioannes (EA1)

JasperRussell (EA1)

Jenos (EA1)

Kale (EA1)

KannH (EA1)

Klimt (EA1)

Knox (EA1)

Kurt1 (EA1)

Leafus (EA1)

Lovelyunicorn (EA1)

Ludgate (EA1)

MaeLinda (EA1)

ManRay (EA1)

Martin (EA1)

McGalleon (EA1)

MillyPhilly (EA1)

MonChoix (EA1)

MrWorldwide (EA1)

Nagem (EA1)

Nattles (EA1)

Oats (EA1)

Oxtober96 (EA1)

Papafritta (EA1)

ParleG (EA1)

Peep (EA1)

Peppino (EA1)

Pherferi (EA1)

Phireproof (EA1)

PhredFlintston (EA1)

Phriends (EA1)

Pocket (EA1)

PuppyEggo (EA1)

Raccoon (EA1)

Raptor (EA1)

Redfield (EA1)

Renzie (EA1)

Riyhil (EA1)

Robinson (EA1)

Schnapsidee (EA1)

Sedgewig (EA1)

Shee (EA1)

SJay (EA1)

SonOfLevi (EA1)

Stanktossa (EA1)

StingRay (EA1)

Stormbreaker8 (EA1)

Strathdee (EA1)

Superfresh (EA1)

TatarkaPM (EA1)

Teagan (EA1)

TeddyBear (EA1)

Tenda (EA1)

Thompsone (EA1)

Thorongil (EA1)

TinSulphur (EA1)

Velene (EA1)

WildNOut (EA1)

Winzigespinne (EA1)

Zada (EA1)

Juanyo (EA10)

Mandalorian (EA10)

Nucci (EA10)

Quartz (EA10)

YuuY (EA10)

HerculesXL (EA11)

Ixel (EA11)

Andromedas (EA2)

ColaCorta (EA2)

Eleri (EA2)

Finny (EA2)

Glamour (EA2)

MCubed (EA2)

Sansa (EA2)

Saratos (EA2)

Casey (EA3)

Pajaza (EA3)

Pikmin (EA3)

Bustleton (EA4)

Golden (EA4)

Kauala (EA4)

Koji (EA4)

Lucky3 (EA4)

Pherbot (EA4)

PrincePhergus (EA4)

Sinatra (EA4)

Alakazam (EA5)

Hasitha (EA5)

Librie (EA5)

Nebulous (EA5)

Neferthena (EA5)

QuadZero (EA5)

Zayuliv (EA5)

Zepp (EA5)

Chepli (EA6)

Jemerald (EA6)

Juicer (EA6)

KatChan (EA6)

Luna18 (EA6)

WilliamStrong (EA6)

Theresita (EA7)

Schubert (EA8)

Cheeto1 (EA9)

ChickenKing (EA9)

GaeCeo (EA9)

Annotating InstitutionSt. Louis Community College
Annotation StatusIn GenBank
Plaque NotesDuring the purification process plaques varied in size and number. The plaques were always temperate turbid and often displayed a surrounding halo.
MorphotypeSiphoviridae
Number of tRNAs1
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON260823
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload