Microbacterium phage MrWorldwide
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Detailed Information for Phage MrWorldwide
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByKayla Sweeney and Will Grillo
Year Found2019
Location FoundPhiladelphia, PA United States
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature24°C
GPS Coordinates39.945333 N, 75.207056 W Map
Discovery NotesIn the University of the Sciences Flower Garden located in the Quad area, next to the path. About a foot away from foot traffic and about fifty feet from the Wilson Dining Hall. Seems to have been recently mulched.
Naming NotesOriginally had a different name, but was taken in the data base.
Mr. Worldwide inspiration came from a helpful Teaching Assistant that was always willing to be a guide during the phage isolation process.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage560
Genome length (bp)41810
Character of genome endsCircularly Permuted
GC Content63.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Other Cluster Members

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Mercedes (None)

AlexAdler (EA1)

Alyxandracam (EA1)

Antoinette (EA1)

Aubergine (EA1)

AxiPup (EA1)

Baines (EA1)

Balsa (EA1)

Bandik (EA1)

BeautPeep30 (EA1)

BeeBee8 (EA1)

Benjalauren (EA1)

BigRedClifford (EA1)

BonesMcCoy (EA1)

Bonino (EA1)

Calix (EA1)

Chamuel (EA1)

Christoph (EA1)

Clancy (EA1)

Convict (EA1)

Dave (EA1)

Den3 (EA1)

Dothraki (EA1)

Espinosa (EA1)

Etna (EA1)

Etta (EA1)

Gargoyle (EA1)

Gelo (EA1)

Gershwin (EA1)

Greys (EA1)

Gubbabump (EA1)

Hamlet (EA1)

HanSolo (EA1)

Ilzat (EA1)

Ioannes (EA1)

Jenos (EA1)

Kale (EA1)

Klimt (EA1)

Knox (EA1)

Kurt1 (EA1)

Leafus (EA1)

Lovelyunicorn (EA1)

Ludgate (EA1)

MaeLinda (EA1)

ManRay (EA1)

Martin (EA1)

McGalleon (EA1)

MillyPhilly (EA1)

MonChoix (EA1)

MrWorldwide (EA1)

Nagem (EA1)

Nattles (EA1)

Oats (EA1)

Oxtober96 (EA1)

Papafritta (EA1)

ParleG (EA1)

Peep (EA1)

Peppino (EA1)

Pherferi (EA1)

Phriends (EA1)

Pocket (EA1)

PuppyEggo (EA1)

Raccoon (EA1)

Raptor (EA1)

Redfield (EA1)

Renzie (EA1)

Riyhil (EA1)

Robinson (EA1)

Schnapsidee (EA1)

Sedgewig (EA1)

Shee (EA1)

SonOfLevi (EA1)

Stanktossa (EA1)

StingRay (EA1)

Superfresh (EA1)

TatarkaPM (EA1)

Teagan (EA1)

TeddyBear (EA1)

Tenda (EA1)

Thorongil (EA1)

TinSulphur (EA1)

Velene (EA1)

WildNOut (EA1)

Winzigespinne (EA1)

Zada (EA1)

Nucci (EA10)

Quartz (EA10)

YuuY (EA10)

Ixel (EA11)

Andromedas (EA2)

ColaCorta (EA2)

Eleri (EA2)

Finny (EA2)

Glamour (EA2)

MCubed (EA2)

Sansa (EA2)

Casey (EA3)

Pajaza (EA3)

Pikmin (EA3)

Bustleton (EA4)

Golden (EA4)

Koji (EA4)

Lucky3 (EA4)

Pherbot (EA4)

PrincePhergus (EA4)

Sinatra (EA4)

Alakazam (EA5)

Nebulous (EA5)

Neferthena (EA5)

QuadZero (EA5)

Zayuliv (EA5)

Zepp (EA5)

Chepli (EA6)

Theresita (EA7)

Schubert (EA8)

ChickenKing (EA9)

GaeCeo (EA9)

Annotating InstitutionFayetteville State University
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesPlaques appeared to be extremely small and fill the entire plate. Plaques appeared to be very turbid and cloudy, with a small bullseye in the middle of the plaques when closely looked at on the bacterial lawn.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.8*10^8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload