Microbacterium phage ChickenKing
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Detailed Information for Phage ChickenKing
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByLucas Polack
Year Found2018
Location FoundBaltimore, MD USA
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates39.331667 N, 76.62 W Map
Discovery NotesFound right in front of a sculpture of a penguin in an art garden. Using enriched isolation I obtained my plaque in small spread out clear spots.
Naming NotesI believed the sculpture was a chicken and needed a mascot for my phage, so I named it ChickenKing
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 30, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1068
Genome length (bp)39924
Character of genome endsCircularly Permuted
GC Content63.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA9
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionJohns Hopkins University
Annotation StatusAwaiting_QC
Plaque NotesAs mentioned before, I began to discover a phage that created spread out clear spots that were very small. As isolation proceeded, the plaques became more concentrated and somehow created larger clear spots. After obtaining what looked like different phages on a single plaque, I performed serial dilutions of each plaque I thought was unique and used one webbed plate I obtained from one of the phages. THE PICTURE CHOSEN WAS MY WEBBED PLATE USED FOR EM. I DID NO
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 86
Pitt Freezer Box Grid# C12
Available Files
Plaque PictureDownload
EM PictureDownload