Microbacterium phage Bustleton
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Detailed Information for Phage Bustleton
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByAmy Varghese, Kumal Siddiq, Isabella Pappano
Year Found2018
Location FoundPhiladelphia, PA United States of America
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature20°C
GPS Coordinates40.097566 N, 75.025998 W Map
Discovery NotesThe soil was obtained on September 24th, 2018 around. 7:42 pm. The soil was wet and it was raining when the soil was collected. The location of the collection site was near the roots of a tree.
Naming NotesThe name "Bustleton" comes from the origin location of the phage, namely the street that runs adjacent to the street on which the soil was obtained. Bustleton Avenue is a well-known street in Philadelphia.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 5, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1137
Genome length (bp)39184
Character of genome endsCircularly Permuted
GC Content64.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA4
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe phage appears to have a clear morphology and small-sized plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Bustleton_1

Bustleton_1

Bustleton_2
(terminase)

Bustleton_2

Bustleton_3
(portal protein)

Bustleton_3

Bustleton_4
(capsid maturation protease)

Bustleton_4

Bustleton_5
(scaffolding protein)

Bustleton_5

Bustleton_6
(major capsid protein)

Bustleton_6

Bustleton_7

Bustleton_7

Bustleton_8

Bustleton_8

Bustleton_9

Bustleton_9

Bustleton_10

Bustleton_10

Bustleton_11

Bustleton_11

Bustleton_12

Bustleton_12

Bustleton_13

Bustleton_13

Bustleton_14
(major tail protein)

Bustleton_14

Bustleton_15
(tail assembly chaperone)

Bustleton_16
(tail assembly chaperone)

Bustleton_15

Bustleton_16

Bustleton_17
(tape measure protein)

Bustleton_17

Bustleton_18
(minor tail protein)

Bustleton_18

Bustleton_19
(minor tail protein)

Bustleton_19

Bustleton_20

Bustleton_20

Bustleton_21

Bustleton_21

Bustleton_22

Bustleton_22

Bustleton_23
(minor tail protein)

Bustleton_23

Bustleton_24
(lysin A)

Bustleton_24

Bustleton_25
(membrane protein)

Bustleton_25

Bustleton_26
(holin)

Bustleton_26

Bustleton_27

Bustleton_27

Bustleton_28

Bustleton_28

Bustleton_29

Bustleton_29

Bustleton_30

Bustleton_30

Bustleton_31
(RecA-like DNA Recombinase)

Bustleton_31

Bustleton_32
(nuclease)

Bustleton_32

Bustleton_33

Bustleton_33

Bustleton_34
(AAA-ATPase)

Bustleton_34

Bustleton_35

Bustleton_35

Bustleton_36
(exonuclease)

Bustleton_36

Bustleton_37
(DNA polymerase I)

Bustleton_37

Bustleton_39

Bustleton_39

Bustleton_40
(DNA helicase)

Bustleton_40

Bustleton_41

Bustleton_41

Bustleton_42

Bustleton_42

Bustleton_43
(MazG-like nucleotide pyrophosphohydrolase)

Bustleton_43

Bustleton_44

Bustleton_44

Bustleton_45
(thymidylate kinase)

Bustleton_45

Bustleton_46

Bustleton_46

Bustleton_47

Bustleton_47

Bustleton_48
(thymidylate synthase)

Bustleton_48

Bustleton_49

Bustleton_49

Bustleton_50

Bustleton_50

Bustleton_51

Bustleton_51

Bustleton_52

Bustleton_52

Bustleton_53

Bustleton_53

Bustleton_54

Bustleton_54

Bustleton_55

Bustleton_55

Bustleton_56

Bustleton_56

Bustleton_57

Bustleton_57

Bustleton_58

Bustleton_58

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK814762
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 81
Pitt Freezer Box Grid# H5
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload