| Detailed Information for Phage Andromedas |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Piper Stanley |
| Year Found | 2017 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.3167 N, 83.1648 W Map |
| Discovery Notes | I attempted to isolate a phage 3 times from Cullowhee Creek before Dr. Wallen made an enrichment and allowed us to use it. This phage came from a shared enrichment with Phage Culver. |
| Naming Notes | My very first pet was named Andromeda. My uncle helped me pick out the puppy from the litter, and my father helped me pick out the name (since I was 4). Andromeda passed away a few years ago, and this is my homage to her. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 15, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2474 |
| Genome length (bp) | 40494 |
| Character of genome ends | Circularly Permuted |
| GC Content | 62.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were neither large nor small, however they were plentiful. My titer ended up being 1.4 x 10^11 pfu/mL. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH590606 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
68 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
