| Detailed Information for Phage Antoinette |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gabrielle Gentile |
| Year Found | 2016 |
| Location Found | Saint Clairsville, OH USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.077045 N, 80.925465 W Map |
| Discovery Notes | Found near an abandoned automotive service on National Road. The soil was taken from a grassy area mixed with gravel from the parking lot. The weather was partly cloudy and sunny. |
| Naming Notes | Named after the Queen of France, Marie Antoinette.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4935 |
| Genome length (bp) | 41858 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Growth of mesas and lysogeny efficiency using phage-seeded plates was attempted with this phage by Audrey Jonas. This phage does not make a lysogen. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Antoinette forms clears plaques that are approximately 2mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH045565 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
