| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Sara Hughes and Mahnoor Siddiqui |
| Year Found | 2019 |
| Location Found | Langhorne, PA United States of America |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.13534 N, 74.8908 W Map |
| Discovery Notes | Discovered Next to a barbecue grill in a suburban area. The soil was moist, and it was dug up about 10 cm below the ground. The temperature outside was about 25 degrees Celsius when the soil was collected. |
| Naming Notes | We chose this name for our Phage due to the fact that “Burrito” was already taken as name for another Phage in the database. We found that this name was very cute as catchy, and it was the first thing to come to our minds. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 40 |
| Genome length (bp) | 41417 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCCGGCA |
| GC Content | 66.5% |
| Sequencing Notes | This phage was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaques are about 0.635 cm in both heigh and width. They are both Turgid and Bulls-eye. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT639649 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.4X10^6 PFU/mL |
| Pitt Freezer Box# |
98 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
