| Detailed Information for Phage Bustleton |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Amy Varghese, Kumal Siddiq, Isabella Pappano |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 20°C |
| GPS Coordinates | 40.097566 N, 75.025998 W Map |
| Discovery Notes | The soil was obtained on September 24th, 2018 around. 7:42 pm. The soil was wet and it was raining when the soil was collected. The location of the collection site was near the roots of a tree. |
| Naming Notes | The name "Bustleton" comes from the origin location of the phage, namely the street that runs adjacent to the street on which the soil was obtained. Bustleton Avenue is a well-known street in Philadelphia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 5, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1137 |
| Genome length (bp) | 39184 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage appears to have a clear morphology and small-sized plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 57 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK814762 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
81 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
