| Detailed Information for Phage ChickenKing |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Lucas Polack |
| Year Found | 2018 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.331667 N, 76.62 W Map |
| Discovery Notes | Found right in front of a sculpture of a penguin in an art garden. Using enriched isolation I obtained my plaque in small spread out clear spots. |
| Naming Notes | I believed the sculpture was a chicken and needed a mascot for my phage, so I named it ChickenKing |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 30, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1068 |
| Genome length (bp) | 39924 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA9 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Johns Hopkins University |
| Annotation Status | In GenBank |
| Plaque Notes | As mentioned before, I began to discover a phage that created spread out clear spots that were very small. As isolation proceeded, the plaques became more concentrated and somehow created larger clear spots. After obtaining what looked like different phages on a single plaque, I performed serial dilutions of each plaque I thought was unique and used one webbed plate I obtained from one of the phages. THE PICTURE CHOSEN WAS MY WEBBED PLATE USED FOR EM. I DID NO |
| Morphotype | Siphoviridae |
| Number of Genes | 57 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN310543 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
86 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
