Detailed Information for Phage Clancy |
Discovery Information |
Isolation Host | Microbacterium foliorum NRRL B-24224 |
Found By | Matthew Johnson |
Year Found | 2018 |
Location Found | La Grange, KY USA |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 38.395926 N, 85.437872 W Map |
Discovery Notes | Soil Sample was mud taken from Reformatory Lake in La Grange, Kentucky. |
Naming Notes | I named the phage after my favorite author, Tom Clancy. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 28, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1596 |
Genome length (bp) | 41555 |
Character of genome ends | Circularly Permuted |
GC Content | 63.4% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | EA |
Subcluster | EA1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Western Kentucky University |
Annotation Status | In GenBank |
Plaque Notes | The plaques were very turbid and through isolation got smaller. Tests were performed to make sure the different sized plaques were the same phage.It was very hard to purify to one plaque. Plaques also had large halos surrounding them. |
Morphotype | Siphoviridae |
Number of Genes | 62 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK967400 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
4.5x10^9 |
Pitt Freezer Box# |
88 |
Pitt Freezer Box Grid# |
E5 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |