| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | EverythingBagel |
| Found By | V Burlak, K Brown, V Nguyen, T Flink |
| Year Found | 2024 |
| Location Found | Amherst, MA United States |
| Finding Institution | University of Massachusetts Amherst |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.39439 N, 72.52515 W Map |
| Discovery Notes | This sample was found in a moist patch of soil near a brick building. Some surrounding vegetation such as grass and weeds were also found. |
| Naming Notes | We named our phage EverythinBagel, in reference to the movie Everything Everywhere all at Once. To obtain a phage, we had to get extra samples of soil from all sorts of places, having to isolate and purify all the samples at once. So, we figured EverythinBagel was a fitting name to represent this process. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2048 |
| Genome length (bp) | 61534 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 201 bp |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaques were found to be clear, bullseye, and about 1 mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PX071748 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
206 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| GenBank File for Phamerator | Download |
