| Detailed Information for Phage Golden |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4333 N, 79.9361 W Map |
| Discovery Notes | This phage was isolated from the plant Solidago spp. collected by Dr. Tia-Lynn Ashman. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius. Purification and amplification was completed by Kira Zack. |
| Naming Notes | Golden refers to the golden color of Solidago spp. flowers. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 14, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1675 |
| Genome length (bp) | 39640 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.1% |
| Sequencing Notes | originally sequenced as MF46, renamed Golden |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and mostly small (a few large plaques also seen). |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925343 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
8 |
| Pitt Freezer Box Grid# |
A3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
