| Detailed Information for Phage Jingles |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Lauren Jingles |
| Year Found | 2024 |
| Location Found | San Antonio, TX United States |
| Finding Institution | University of Texas at San Antonio |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.58393 N, 98.61852 W Map |
| Discovery Notes | I discovered the phage near the Rowdy statue on campus, collecting soil that was relatively moist due to the sprinkler system and surrounded by plant foliage. It is currently cricket season in San Antonio, and while I am uncertain if their presence impacts the soil, it is worth noting their abundance in the area. The sample was taken from approximately 1.5 inches beneath the surface layer, which was covered with wood chips. The soil itself was easily compacted into the conical tube. |
| Naming Notes | I am naming the phage after myself, and my name is Lauren Jingles. I believe that the name is fun and would not only be amusing but easy to say aloud at conferences. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 4553 |
| Genome length (bp) | 39281 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Texas at San Antonio |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | "During my first round of serial dilutions, the phage exhibited clear lytic activity, with multiple turbid clearings of varying diameters. After several rounds, I successfully isolated the phage when it produced a large plaque with no growth nearby. In the spot titer assay, the phage exceeded expectations by creating more than the three plaques we aimed for, resulting in a complete clearing. |
| Morphotype | Siphoviridae |
| Number of Genes | 56 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
