Microbacterium phage Jingles
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Detailed Information for Phage Jingles
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByLauren Jingles
Year Found2024
Location FoundSan Antonio, TX United States
Finding InstitutionUniversity of Texas at San Antonio
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates29.58393 N, 98.61852 W Map
Discovery NotesI discovered the phage near the Rowdy statue on campus, collecting soil that was relatively moist due to the sprinkler system and surrounded by plant foliage. It is currently cricket season in San Antonio, and while I am uncertain if their presence impacts the soil, it is worth noting their abundance in the area. The sample was taken from approximately 1.5 inches beneath the surface layer, which was covered with wood chips. The soil itself was easily compacted into the conical tube.
Naming NotesI am naming the phage after myself, and my name is Lauren Jingles. I believe that the name is fun and would not only be amusing but easy to say aloud at conferences.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notes"During my first round of serial dilutions, the phage exhibited clear lytic activity, with multiple turbid clearings of varying diameters. After several rounds, I successfully isolated the phage when it produced a large plaque with no growth nearby. In the spot titer assay, the phage exceeded expectations by creating more than the three plaques we aimed for, resulting in a complete clearing.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload